Daniel Zhang Email & Phone Number

Philadelphia, PA, US

Rahway, New Jersey, US

• Performed HIV infection of T-cells and whole population of primary human peripheral blood mononuclear cells (PBMC) under BSL2+ laboratory conditions to quantify cell infection and NK cell antibody directed cellular.
• Reformatted HIV ADCC model to 384-well format with potential to characterize antibody candidates’ ADCC and neutralization ability in a high throughput manner, potentially develop a similar 3D model
• Conducted cross-titration study on donor plasma, antibody/small molecule and protein precursor synergy study for effective neutralization of HIV infected CD4+ T-cells to explore possible new/personalized therapies for.
• Employed high fluorescence techniques to report assay effectiveness and further increased the sensitivity of the model using Gag/p24 antigen ELISA developed in house and modified to fit the new 384-well format
• Facilitated Surface Plasmon Resonance/Bio-layer interferometry (SPR/BLI) to study surface binding dynamics of antibodies on CD4, alongside with automatic platform (Biomek) for high throughput ELISA assay development
• Current assay has been used for multiple small molecule/antibody screening projects for potential HIV cure, and successfully selected outstanding candidates for more informed choices during animal trail

Philadelphia, PA, US

• Project: Src Family Kinase (SFK)/spleen tyrosine kinase (Syk) inhibition for primary/secondary platelet deposition
• Interrogated the difference of platelet activation/deposition inhibited by Syk/SFK inhibitor with/without thrombin
• Differentiated the effect of primary and secondary platelet deposition during clotting with inhibition of Syk/SFK
• Quantified the platelet activity at primary and secondary platelet deposition with phosphatidylserine
• Proposed a possible cell signaling pathway of platelet through glycoprotein VI (GPVI), SFK and Syk to explain undesirable bleeding in current leukemia treatmentsProject: Direct inhibition of glycoprotein VI (GPVI) for.
• Coordinated with Dr. Nieswandt for testing proprietary human GPVI blocking antibody Fab in our microfluidic assay

Philadelphia, PA, US

• Participated in informational interviews with members of the Infectious Disease & Vaccine department and collaborating departments
• Connected with junior and senior staff to gain perspectives on work duties, such target identification, chemical screens, vaccine design, bioengineering, immunological assays and resistance testing, formulations, and.
• Enhanced skills in professional development, leadership, and networking strategies in pharmaceutical industry
• Established relationships and networks with advanced degree holding professionals through one-on-one mentoring
• Attended seminars for resume polishing, interview skills, and career goal setting to facilitate future development

Philadelphia, PA, US

• Prepared and used equipment for cell culture, PCR and DNA sequencing, chromatography, Western Blot, etc.
• Graded homework, lab reports and quizzes, then provided one-on-one feedbacks for 22 individual students

Brentford, Middlesex, GB

• Primary Project: Multicellular Organotypic 3D breast tumor model
• Adapted a scalable complex 3D in vitro model, which included organotypic tumor microenvironment (TME) features and high throughput system (HTS) technologies, toward a breast cancer context of use
• Led the assay development and recommended optimized cell number and ratio, culture duration and imaging endpoints for the breast tumor complex model with three different tumor cell lines
• Performed lentiviral transfection and selection to fluorescently-tag multiple cell lines for high content imaging
• Developed thorough, detailed protocols and meticulously documented studies in an electronic lab notebook
• Conducted thorough literature search reviews to recommend a “state of the art” update in breast tumor organoid and tissue engineered models for assay internalizationEmerging Project: Immunocompetent lung air-liquid.

Philadelphia, PA, US

• Investigated technical details in synthesizing, manufacturing and storage of hydrocortisone sodium succinate (HSS) production for an innovative drug delivery device
• Assessed the production details and market sizes of antipsychotics, IVF and monoclonal antibodies and evaluated the significance of these potential markets
• Analyzed the details of automated lyophilization with other company associates
• Surveyed other medical startups for potential competition, market entry and exit strategies
• Investigated the feasibility of facilitating ENHANZE® drug delivery technology in our device for monoclonal antibodies

• Explored the general process and details in consulting cases for company evaluation in healthcare industry
• Investigated sections and approaches for market analysis in consulting cases in healthcare industry
• Learned and evaluated proper pricing strategies for healthcare products and services
• Applied and showcased the skills and knowledge gained from these series through a real case capstone project

• Explored the market size in different regions for patented, innovative devices from startups
• Evaluated groups of stake holders for these devices and regulations at various markets
• Formulated a scoring matrix that ranked other potential markets to prioritize strategic goals
• Conducted primary and secondary research of stake holders’ concerns for cost and pricing analysis
• Patented devices are from: In Vitro Diagnostic Solutions, LLC. and SOLUtion Medical, LLC.

Bethlehem, PA, US

• Understood composition, process and requirements in sterile drug production
• Managed capital and operational cost of the production process
• Specialized in process design of lyophilization based on condition requirements from clients
• Presented process design, control scheme and cost analysis to peer, faculty and industrial advisor

Bethlehem, PA, US

• Graded homework, lab reports, quizzes, and midterm exams for undergraduate level classes
• Held office hours, exam preparation sessions and recitations

Bethlehem, PA, US

• Prepared samples of silica particles with various sizes for membrane manufacturing
• Used Small Angle X-Ray Scattering (SAXS) machine to evaluate the distribution of particle sizes
• Studied the permeable features of membrane with different particle sizes

Bethlehem, PA, US

• Quantitative numerical prediction of product composition under different reaction conditions using MATLAB
• Setup and test operation of reactor, qualitative analysis of the difference between actual product and numerical prediction
• Operation of combustible and toxic gas tanks with certification
• Device was used since Fall 2017 for Unit Ops Lab in Lehigh University

Bethlehem, PA, US

• Studied the principle of Fourier Transform Infrared (FTIR) Microscopy
• Designed and manufactured a durable, accurate yet small size FTIR Microscope with 3D printing, with potential for patenting

Doctor Of Philosophy - Phd, Chemical And Biomolecular Engineering

Bachelor Of Science - Bs, Chemical And Biomolecular Engineering