Daniel C. L. Email & Phone Number

Seattle, Washington, United States

• Led and executed customer-focused projects, validating and screening new drugs (small molecules, antisense oligonucleotides, adenovirus) developed by pharmaceuticals for skeletal muscle diseases using state-of-the-art.
• Managed resources, planned experiments, maintained detailed records on electronic lab notebooks, and presented findings for customer projects, enhancing business development and customer satisfaction.
• Automated cellular and tissue imaging tasks, including high-throughput immunofluorescence, cellular assays, live-cell imaging, and whole-section histology, boosting operational efficiency.
• Pioneered the use of CellProfiler pipelines for myoblast purity analysis, myofiber quantification, and other cellular assays, improving data reliability and processing speed.

Seattle, Washington, United States

• Developed and managed a comprehensive imaging system/database, setting a new standard for high-quality scientific imaging within the team

Seattle, Washington, United States

• Established lab management systems for ordering, inventory, liquid nitrogen storage, and environmental monitoring of freezers, refrigerators, and incubators.
• Implemented Standard Operating Procedures (SOPs), templates, and macros for new research processes and experiments, enhancing lab efficiency.

Seattle, Washington, United States

• Led multidisciplinary research projects, achieving key milestones and publications in organ-specific endothelial research.
• Developed immortalized human kidney endothelial cell lines, improving organ specificity in kidney research and replacing commercially available HUVEC.
• Served as the primary expert in virus production, specializing in transfection and transduction techniques.
• Established efficient record-keeping systems, becoming a key resource for colleagues seeking data from lab notebooks and databases.
• Characterized and analyzed genotypes and phenotypes of diverse cell lines (patient-derived, immortalized, and stem-cell derived) using flow cytometry, qRT-PCR, Western blot, ELISA, and immunofluorescent staining.
• Differentiated human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) into cardiomyocytes, endothelial cells, and kidney organoids.

Seattle, Washington, United States

• Designed nanopatterned cantilever assays for cardiac preclinical screening.
• Cultured NRVM, C2C12, and hESC-CMs.
• Differentiated hESCs and iPSCs into cardiomyocyte using feeder-free method.
• Analyzed images and videos using ImageJ and Matlab.
• Fabricated biomaterial substrates using nanolithography and spin coating using PDMS and PLGA.

Taipei City, Taiwan

Master Of Science (M.S.), Bioengineering And Biomedical Engineering

• Master's Thesis: 𝗔 𝗡𝗮𝗻𝗼𝗽𝗮𝘁𝘁𝗲𝗿𝗻𝗲𝗱 𝗖𝗮𝗻𝘁𝗶𝗹𝗲𝘃𝗲𝗿 𝗗𝗲𝘃𝗶𝗰𝗲 𝗳𝗼𝗿 𝗔𝘀𝘀𝗮𝘆𝗶𝗻𝗴 𝗖𝗼𝗻𝘁𝗿𝗮𝗰𝘁𝗶𝗹𝗲 𝗣𝗿𝗼𝗽𝗲𝗿𝘁𝗶𝗲𝘀 𝗼𝗳 𝗦𝘁𝗲𝗺 𝗖𝗲𝗹𝗹-𝗗𝗲𝗿𝗶𝘃𝗲𝗱 𝗖𝗮𝗿𝗱𝗶𝗼𝗺𝘆𝗼𝗰𝘆𝘁𝗲𝘀 • TA for.

Bachelor Of Science (Bs), Bioengineering And Biomedical Engineering, 3.74/4.0

Activities and Societies: Tau Beta Pi, UW Photography Club• Graduated with 𝗖𝘂𝗺 𝗟𝗮𝘂𝗱𝗲 𝘄𝗶𝘁𝗵 𝗛𝗼𝗻𝗼𝗿𝘀 𝗶𝗻 𝗕𝗶𝗼𝗲𝗻𝗴𝗶𝗻𝗲𝗲𝗿𝗶𝗻𝗴 • Received 𝗠𝗮𝗿𝘆 𝗚𝗮𝘁𝗲.