Daniel C. L. Email & Phone Number

Seattle, Washington, United States

• Led and executed customer-focused projects, validating and screening new drugs (small molecules, antisense oligonucleotides, adenovirus) developed by pharmaceuticals for skeletal muscle diseases using state-of-the-art Mantarray™, Nautilai™, and other advanced biosystems.• Managed resources, planned experiments, maintained detailed records on electronic lab notebooks, and presented findings for customer projects, enhancing business development and customer satisfaction.• Automated cellular and tissue imaging tasks, including high-throughput immunofluorescence, cellular assays, live-cell imaging, and whole-section histology, boosting operational efficiency.• Pioneered the use of CellProfiler pipelines for myoblast purity analysis, myofiber quantification, and other cellular assays, improving data reliability and processing speed.

Seattle, Washington, United States

• Developed and managed a comprehensive imaging system/database, setting a new standard for high-quality scientific imaging within the team

Seattle, Washington, United States

• Established lab management systems for ordering, inventory, liquid nitrogen storage, and environmental monitoring of freezers, refrigerators, and incubators.• Implemented Standard Operating Procedures (SOPs), templates, and macros for new research processes and experiments, enhancing lab efficiency.

Seattle, Washington, United States

• Led multidisciplinary research projects, achieving key milestones and publications in organ-specific endothelial research.• Developed immortalized human kidney endothelial cell lines, improving organ specificity in kidney research and replacing commercially available HUVEC. • Served as the primary expert in virus production, specializing in transfection and transduction techniques.• Established efficient record-keeping systems, becoming a key resource for colleagues seeking data from lab notebooks and databases.• Characterized and analyzed genotypes and phenotypes of diverse cell lines (patient-derived, immortalized, and stem-cell derived) using flow cytometry, qRT-PCR, Western blot, ELISA, and immunofluorescent staining.• Differentiated human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) into cardiomyocytes, endothelial cells, and kidney organoids.• Led a kinase activity assay project, using high-throughput imaging and analysis to screen the effects of over 30 kinases in human organ-specific endothelial cell lines.

Seattle, Washington, United States

• Designed nanopatterned cantilever assays for cardiac preclinical screening.• Cultured NRVM, C2C12, and hESC-CMs.• Differentiated hESCs and iPSCs into cardiomyocyte using feeder-free method.• Analyzed images and videos using ImageJ and Matlab.• Fabricated biomaterial substrates using nanolithography and spin coating using PDMS and PLGA.

Taipei City, Taiwan

Master Of Science (M.S.), Bioengineering And Biomedical Engineering

• Master's Thesis: 𝗔 𝗡𝗮𝗻𝗼𝗽𝗮𝘁𝘁𝗲𝗿𝗻𝗲𝗱 𝗖𝗮𝗻𝘁𝗶𝗹𝗲𝘃𝗲𝗿 𝗗𝗲𝘃𝗶𝗰𝗲 𝗳𝗼𝗿 𝗔𝘀𝘀𝗮𝘆𝗶𝗻𝗴 𝗖𝗼𝗻𝘁𝗿𝗮𝗰𝘁𝗶𝗹𝗲 𝗣𝗿𝗼𝗽𝗲𝗿𝘁𝗶𝗲𝘀 𝗼𝗳 𝗦𝘁𝗲𝗺 𝗖𝗲𝗹𝗹-𝗗𝗲𝗿𝗶𝘃𝗲𝗱 𝗖𝗮𝗿𝗱𝗶𝗼𝗺𝘆𝗼𝗰𝘆𝘁𝗲𝘀 • TA.

Bachelor Of Science (Bs), Bioengineering And Biomedical Engineering, 3.74/4.0

Activities and Societies: Tau Beta Pi, UW Photography Club• Graduated with 𝗖𝘂𝗺 𝗟𝗮𝘂𝗱𝗲 𝘄𝗶𝘁𝗵 𝗛𝗼𝗻𝗼𝗿𝘀 𝗶𝗻 𝗕𝗶𝗼𝗲𝗻𝗴𝗶𝗻𝗲𝗲𝗿𝗶𝗻𝗴 • Received 𝗠𝗮𝗿𝘆 𝗚𝗮𝘁𝗲.