Danya Ariel Dean, Phd Email & Phone Number

Cambridge, Massachusetts, United States

• Targeted and untargeted LC-MS/MS (QQQ, QTOF, Orbitrap) based metabolomics for early drug discovery, target validation and biomarker research in oncology, immunology, and neuroscience • Benchmarking and method development of microflow LC-MS technology for sample limited and low abundant metabolite analysis • Method development of metabolite panels to enhance team’s capability of small molecule coverage • Development of structured data analysis workflow using vendor agnostic tools… Show more • Targeted and untargeted LC-MS/MS (QQQ, QTOF, Orbitrap) based metabolomics for early drug discovery, target validation and biomarker research in oncology, immunology, and neuroscience • Benchmarking and method development of microflow LC-MS technology for sample limited and low abundant metabolite analysis • Method development of metabolite panels to enhance team’s capability of small molecule coverage • Development of structured data analysis workflow using vendor agnostic tools for batch extraction of metabolites, statistical analysis, and visualization to create an efficient data analysis pipeline to increase result turn around for stakeholders• Developed highthroughput metabolite extraction method using Biomek Liquid Handler increasing team sample analysis bandwidth• Extensive literature research to connect metabolomics findings with biological evidence using BenchSci• Serve as metabolomics functional lead for essential projects and programs (subject matter expert to develop scientifically sound study designs, conduct analysis and data interpretation on cross functional project teams) Show less

Boston, Massachusetts, United States

• Protein stability characterization using DSF, Flow Cytometry, and SEC• Optimization of stress conditions for biologics developability risk assessments• Formulation development for biologic molecules to preserve stability and activity

Graduate Research Assistant, Advisor: Dr. Laura-Isobel McCallDissertation Title: Fundamental and Translational Applications of LC-MS/MS Based Metabolomics in Infectious DiseasesUse LC-MS based metabolomics to understand infectious disease pathogenesis, metabolism, host-pathogen interactions, and biomarkers for novel diagnostic methods• Conducted BSL-2 cell culture (mammalian cell lines, primary and secondary cells) to infect mice with pathogen (parasite/virus) • Infected mice… Show more Graduate Research Assistant, Advisor: Dr. Laura-Isobel McCallDissertation Title: Fundamental and Translational Applications of LC-MS/MS Based Metabolomics in Infectious DiseasesUse LC-MS based metabolomics to understand infectious disease pathogenesis, metabolism, host-pathogen interactions, and biomarkers for novel diagnostic methods• Conducted BSL-2 cell culture (mammalian cell lines, primary and secondary cells) to infect mice with pathogen (parasite/virus) • Infected mice (intraperitoneal, subcutaneous injections) with pathogen to understand host-pathogen interactions and disease pathogenesis in Influenza Virus and Chagas Disease• Conducted necropsies, blood (tail and saphenous vein), tissue, saliva, urine, and feces collection to conduct DNA & RNA Extraction, ELISA, Western Blot, RT-qPCR, and other in vivo and in vitro experimentation to identify novel treatments/biomarkers: for Chagas Disease to determine effects of carnitine on cardiac metabolism for treatment efficacy/safety and biomarkers for improved diagnostics , for understanding disease pathogenesis of influenza virus in the lung • Untargeted LC-MS (Orbitrap) analysis for identification of differential metabolites utilizing MZmine, Global Natural Product Social Molecular Networking Site (GNPS), Qiime, and Skyline • Conducted complex omics analysis using R and Python to carry out random forest machine learning and for development of visualizations such as volcano/box plots, PCoA plots etc.• Conducted molecular networking using Cytoscape and spatial metabolomics by developing 3D organ models using SketchUp, MeshLab, and ‘ili to determine location, tropism and distribution of pathogen and metabolites• Created publication ready figures using Adobe Illustrator, Inkscape, BioRender, and Prism Show less

Responsible as a qualified graduate student for assisting instructors in conducting classes or research, teaching students, and preparing lectures. Primary instructor for undergraduate laboratory sections indicated above. Supervised students with wet labs, graded lab reports and recitation worksheets. Met with students individually to aid in chemistry related questions and concerns.

Upland, Indiana

• Carried out experiments to ensure the precision and accuracy of several analytical instruments.• Conducted weekly calibration and inspection of instruments. • Engaged with professors to gain skills in using several analytical techniques. • Troubleshot instruments when problems arise to prevent from proceeding in the future.

Upland, Indiana

•Tested experiments to ensure accuracy and assist students in lab•Prepared lab for students, gave instructions for clarity and engaged students in discussion of lab information•Used chemical inventory program (Quartzy) to track chemical use and location of equipment

Upland,Indiana

•Developed a lab procedure for the Forensic Science course using RT-PCR and traditional PCR methods•Compared the use of two DNA profiling kits which implemented PCR techniques•Tested different agarose staining methods

Grand Bahama, The Bahamas

• Worked alongside chemists in Process Development to ensure efficiency of plant procedures by problem solving and developing ideas to increase manufacturing efficacy • Aided in investigations when problems arose and tested for resolutions• Conducted experiments (crystallizations and synthesis) to increase plant productivity and ensure reliable results

Nassau, The Bahamas

• Assisted in investigation of crime cases by using equipment and carried out hands on work• Conducted experiments and concluded results found to write court cases• Worked alongside multiple specialists to gain knowledge of the field to be used later in life

Doctor Of Philosophy - Phd, Chemistry And Biochemistry

Bachelor Of Arts, Chemistry