David Lligé Email and Phone Number

Carrying out the project Biomarkers and combinatorial drug targets for personalized therapy for three major cancers PIE16/00011By simulating tratments in culture, I permformed loss-of-funtion screens in order to identify genes affecting drug sensitivity.Culture cell lines, viability assay, drug testing, gene expression, gene silencing

Immunuhistochemistry, Immunofluorescence, Immunoassay, Flow Cytometry, Confocal Microscopy

Primary cultures of microglia and astrocytes in cortex and cerebellum in mice and ratsOrganotypic culture in rat hippocampal slices.Cytochemistry and Immunohistochemistry techniques.

BIOCHEMISTRY Measurement of mitochondrial electronic transport chain enzyme activities (CI-III, CII+III, CIII, COX, CS) (Shimadzu UV 2401 PC)Measurement of Antioxidant enzyme activities (SOD, GPX, Catalase) ) (Shimadzu UV 2401 PC)Polarographic measurement of oxygen consumption in cell (Oxigraph Plus Hansatech) MOLECULAR BIOLOGY DNA/RNA isolation, PCR, cDNA synthesis, sequencing, cloning, RFLP’s, western blot, southern blot, Microfluidic cards set up. CELL CULTURES Standard techniques of cell culture in eukaryotic cells IMMUNOASSAY ELISA