David Nyer Email & Phone Number

Boston, MA, US

Cambridge, Massachusetts, US

Berkeley, CA, US

• Optimized Caribou’s Type I CRISPR technology for efficient genome editing in therapeutically relevant cell lines.
• Proposed, designed, and executed novel experiments based on primary literature research.
• Onboarded a cell-based assay for detection of off-target editing from the Type I system.
• Trained group members in cell culture and nucleic acid generation.
• Cultured mammalian cell lines (immortalized lines, T-cells, and iPS cells).
• Prepared protein and nucleic acid reagents for transfection into cells, performed transfections, analyzed results through flow cytometry assay, viability assays, fluorescence microscopy, and NGS.

Berkeley, CA, US

Tempe, AZ, US

Maintains human cell cultures and performs experiments based on RNA-seqanalysis, qPCR, and fluorescence microscopy/flow cytometry. Assembles novel DNA constructs using molecular cloning techniques. Trains undergraduate volunteers and coordinates volunteer activities.

Gilbert, AZ, US

Algal strain cultivation and development, flow cytometry and fluorescence microscopy. Researched compounds for antimicrobial treatment of cultures. Developed techniques for identification and characterization of bacterial contaminants using high-throughput instrumentation.

Tempe, AZ, US

Researched the role of cyanobacteria in arid and semi-arid soil crust communities. Studied the metabolomics of genetically modified cyanobacteria to produce higher-quality biofuels.

Reston, VA, US

Assisted in researching and reporting on RCRA-related tasks for US Air Force remediation projects. Conducted historical research on point source groundwater contamination in the greater Phoenix area.

Tempe, AZ, US

Processed soil samples, managed laboratory processes, trained and managed laboratory workers.

Master Of Science (M.S.), Molecular Biology

Bachelor Of Science (B.S.), Biochemistry And Molecular Biology