seqWell.com

Guidance of assay development activities, including technical direction, report preparation and document review.

Responsible for all assay-related aspects of IVD product development pipeline. Built and managed assay development team. Oversaw design, development and optimization of isothermal amplification assay panel for host gene expression analysis. Contributed to non-dilutive funding opportunities and managed reporting to funding agencies. Co-managed CDO's/CMO's. Worked with QA to implement GLP/QMS for laboratory.

Responsible for demonstrating feasibility of isothermal amplification strategy for ultra-rapid host gene expression analysis to be conducted at the point of care. Oversaw development and implementation of gold-standard sample processing pipeline for generating gene expression data on prospectively collected human whole blood specimens.

Responsible for outfitting wet lab for molecular assay development. Performed feasibility studies to down-select technologies for host gene expression analysis at point of care.

Development of mutational analysis assays for applications in oncology. Demonstrated feasibility of ultra-specific primers for SNP and IN/DEL detection from circulating, cell-free DNA. Integrated test method with GeneXpert cartridge. Oversaw pilot program on developing urine as a sample input for circulating biomarker detection.

High throughput screening assay development, hit validation, and lead optimization to identify small molecules capable of modulating the RLR-mediated innate immune response.

Enzymological and cell-based studies aimed at defining the molecular mechanisms of non-self RNA detection and signal transduction used by the innate immune receptor RIG-I to perform cytoplasmic surveillance against pathogenic RNAs deposited in cells by viruses such as HIV and Influenza.

High throughput protein expression, purification, and crystallization for structural studies of HIV budding, chromatin remodeling, and proteosomal protein complexes.

Cloning, expression, purficiation, and photo-kinetic analysis of the blue light-activated bacterial histidine kinase LovK.