David Stiles Email & Phone Number

Gaithersburg, Maryland, United States

Quality team member that drives Quality Control activities associated with biological and molecular assay transfer and qualification. Develop assays using ICH guidelines as your standards. Assist the assay transfer from Process Development team to GMP and has an active role in assay qualification including report generation. Assay development tasks include.

Gaithersburg, Maryland, United States

Quality team member that drives Quality Control activities associated with biological and molecular assay transfer and qualification. Develop assays using ICH guidelines as your standards. Assist the assay transfer from Process Development team to GMP and has an active role in assay qualification including report generation. Assay development tasks include.

Gaithersburg, Maryland, United States

Quality team member that drives Quality Control activities associated with biological and molecular assay transfer and qualification. Develop assays using ICH guidelines as your standards. Assist the assay transfer from Process Development team to GMP and has an active role in assay qualification including report generation. Assay development tasks include.

Rockville, Maryland, United States

• Responsible for training and supervising roughly 10 laboratory technicians. Leverage BSL2+ experience. Perform routine and specialized laboratory procedures, quality control, and quality assurance as outlined in.
• Conducted successful method validation, performance qualification, and reproducibility testing resulting in increased testing throughput of clinical specimens for the detection of Coronavirus.
• Reviewed and released clinical Coronavirus testing results in a complete, timely, and accurate manner after completing standard quality control.

Rockville, Maryland, United States

• Promoted twice in less than a year to positions of increasing responsibility and complexity. Design and managed all aspects of laboratory-based Coronavirus testing. Demonstrated designed and performed qPCR assay.
• Built RT-PCR based testing methodologies for Coronavirus, that is still in use today.
• Trained technical staff, resulting in ramp-up of proficiency and ability for technicians to work independently in the laboratory.
• Conducted numerous lab experiments, achieving notable results: o Implemented viral RNA extraction method of various types of clinical and environmental specimens. o Developed RT-PCR, based testing for the detection and.

Rockville, Maryland, United States

• Processing of samples for the isolation of nucleic acids from various sample types, prep for RT-PCR, and running RT-PCR for COVID-19 testing. Performs tests using PCR, RT-PCR and qRT-PCR to analyze body fluids, tissue.
• Processing of various sample types for the isolation of nucleic acids.
• Prepared viral RNA samples for RT-PCR, based testing for the detection and quantification of SARS- CoV-2 virus.
• Drafted the development of a GLP standard operating procedure for RT-PCR based testing of wastewater sample concentrates and clinical stool specimens.

Rockville, Maryland, United States

• Handle incoming samples for accessioning, organizing, storing, and processing. Processing of samples will include the isolation of nucleic acids from various sample types, prep for RT-PCR, and running RT-PCR for.
• Developed viral RNA extraction method of various types of clinical and environmental specimens.
• Developed RT-PCR, based testing for the detection and quantification of SARS-CoV-2 virus various types of clinical and environmental specimens.

Bethesda, MD

• Complete research objectives and support ongoing research projects in the Department of Transfusion Medicine.
• Helped with the determination of the Genomic coordinates, distribution, and frequency of 120 blood group variants reported in the 1000 Genomes Project.
• Designed, developed and troubleshooted real-time qPCR-based assays for the determination of Blood Donor RHD zygosity and Rh exon copy number determination.
• Co-authored “Genomic coordinates and continental distribution of 120 blood group variants reported by the 1000 Genomes Project” Transfusion. 2018 Nov;58(11):2693-2704.

Bethesda, MD

• Examined research objectives relevant to laboratory goals. Organized, established, and performed experiments in agreement to established protocols. Evaluated data ensuring experimental goal achievement and applied.
• Utilized R to write scripts for descriptive and inferential statistics analysis of large datasets of blood usage metrics in the Department of Transfusion Medicine.
• Visualization of large datasets of metrics and treads to develop new approaches of prospectively genotyping of blood donors with hard to match transfusion patients.
• Co-authored “Two large deletions at either end of the RHD gene extending beyond the gene’s limit and their red cell phenotypes” Kshitij Srivastava, David Alan Stiles, Franz Friedrich Wagner, and Willy Albert Flegel..

Gaithersburg, MD

• Utilized data analysis software packages to furnish qRT-PCR and microarray data analysis services for internal research and development projects and delivery to Gene Logic clients. Established R scripts automating.
• Implemented quality control data analysis and visualization of in-house-generated qRT-PCR and microarray data using R.
• Used robotic liquid handling systems for automated processing of real-time PCR assays and DNA sequencing.

Bethesda, MD

• Collaborated with Senior Investigators on laboratory experiments in support of biomedical research programs and projects. Created, organized, and steered research while serving as expert resource in Molecular Biology.
• Oversaw and qualified post-doctoral fellows, research associates, and other technical or field staff involved in specialized molecular biology laboratory techniques, procedures, and laboratory safety, especially.
• Co-authored “High-Throughput Retina-Array for Screening 93 Genes Involved in Inherited Retinal Dystrophy” David Stiles, Jin Song, Nizar Smaoui, Radha Ayyagari, Sonia Benhamed, Ian M. MacDonald, Stephen P. Daiger, Santa.
• Co-authored “The functional effect of pathogenic mutations in Rab escort protein 1” Y.V. Sergeev, N. Smaoui, R. Sui, D. Stiles, N. Gordiyenko, N. Strunnikova, I.M. MacDonald Mutation Research/Fundamental and Molecular.

Bethesda, MD

• Maintained adherence to experimental procedures, maintained and analyzed records of test results for submission to scientific meetings and journals, and planned research studies to meet experimental objectives..
• Co-authored “A detailed transcript-level probe annotation reveals alternative splicing based microarray platform differences” Joseph C, Lee, David Stiles, Jun Lu, and Margaret C Cam, BMC Genomics 2007, 8:284.
• Co-authored “Long CGG-repeat tracts are toxic to human cells: Implications for carriers of Fragile X permutation alleles” Vaishali Handaa, Deena Goldwater, David Stiles, Margaret Cam, George Poy, Daman Kumaria, and.

Ma, Biology/Biological Sciences, General

Marshall University is a coeducational public research university in Huntington, West Virginia, United States, founded in 1837, and named.

Bs, Biological Sciences

Marshall University is a coeducational public research university in Huntington, West Virginia, United States, founded in 1837, and named.