David Shaya Email & Phone Number

Alameda, California, Us

Sr. Scientist I/II, Lead discovery - developed and executed successful biophysical and biochemical assays for high-throughput screening, effective SAR campaigns and target MOA characterization.♦ Lead discovery project leadership in cross-functional teams, responsible for project aspects from conceptualizing screening strategies to a successful execution of effective SAR campaigns.♦ I collaboratively worked with chemistry and structure biology project leads to meet project needs designing and overseeing the execution of various assays characterization of protein-protein interaction and advancing novel chemical matter.♦ I successfully developed and executed biosensor high throughput screens, leading to generation of advanced leads through structure-enabled hit-to-lead campaigns (weekly SAR) using protein binding/proximity assays such as Surface Plasmon Resonance (SPR), TR-FRET probe displacement, Lumit and AlphaLISA combined with activity based assays such as enzyme kinetics, ADP-Glo, Luminescence, ELISA.♦ I ran focused campaigns to advance and expand chemical scaffolds including SPR-based fragment screen, activity/TR-FRET - based virtual screen and DNA encoded libraries screens (DEL).♦ I successfully implemented automation tools and robotic liquid handlers for accurate and reproducible efficacy/inhibition determination of dozens to enable effective SAR.♦ I was in responsible for data processing and assimilation across various stakeholder to meet project deadline.

South San Francisco, Ca, Us

I Leveraged structure-driven platform to develop novel therapeutic application for high-value drug targets. I played a central role in developing and validating BlueLight’s Second Harmonic Generation (SHG) technology to study protein conformational changes upon ligand application. Subsequently, I successfully executed the first SHG high-throughput screen ran on the platform including protein target expression/purification, quality control and protein labelling.♦ I conceived and executed small molecule screens using the SHG technology including assay development and optimization, HTS application, data analysis and validation.

South San Francisco, Ca, Us

♦ I established large scale protein expression and labeling to generate and QC proteins enabling HTS.♦ I designed and executed orthogonal assays for hit validation including ADP-Glo, DiFMUP and HTRF assay.♦ I biophysically characterized protein targets and developed activity assays for early discovery targets.

South San Francisco, Ca, Us

♦ I worked with external pharma and academic partners on key collaboration projects.♦ I adapted the SHG technology and workflows to characterize novel hard-to-drug targets and membrane protein samples (RAS, GPCR).

South San Francisco, Ca, Us

♦ I launched 2 novel surface products: Phosphatidylserine (PS) and biotinylated surfaces expanding the company applicability portfolio. This included developing model proteins to develop, test and validate these surfaces (used in SHG technology application for RAS and biotinylated targets).♦ Managed construct design, protein expression and purification in close collaboration with CRO.

Los Alamos, Nm, Us

Postdoctoral Research Associate, Structural studies of Multi-Drug-resistance Efflux pumps complexes♦ I screened efflux pumps components cloning and expression optimization to identify high over-expressing, properly folded 24-TM inner membrane transporter constructs leading to crystallizable constructs.♦ Membrane protein engineering and construct optimization generating several crystallizable samples .♦ I established high-yield reproducible purification scheme coupling biophysical assays to test and optimize detergents usage to stabilize membrane protein samples. Isolated trimeric transporter complexes yielding crystals stable over two weeks.♦ Generated samples for EM data collection yielding a low-resolution envelope structure.♦ Crystallization, cryo-protection and data collection of transporter samples leading to a low-resolution protein model corroborating EM data generated.

San Francisco, California, Us

American Heart and Stroke Assoc. Postdoctoral Research Fellow (2011-2013), Structural and Biophysical Investigations of Sodium Channels (membrane spanning proteins)♦ Using innovative protein engineering, I created high over-expressing tetrameric, active ‘pore-protein’ ion channels by removing the voltage sensor and expressing the ion permeation pathway of the channel.♦ I ran medium throughput screen, cloning ion channels orthologs to identify 3 crystallizable constructs.♦ I collected X-ray data (2.7-3.5 A), generated 3D models and solved the structures of 3 Na+ channels.♦ I conducted a comprehensive biophysical and biochemical characterization to QC samples including: blue native PAGE, chemical cross-linking, Size Exclusion Chromatography, Multi Angle Light Scattering (MALS)-RI analysis, CD, thermal denaturation stability assays (DSF), incorporation into lipid vesicles and single molecule electrophysiology.

Ottawa, Ontario, Ca

Structural and Kinetic Studies of Glycosaminoglycan (GAG) Degrading Enzymes♦ Structural and functional analysis of 3 heparin-degrading enzymes. I solved the 3D structures of 3enzymes mastering protein expression, purification, crystallization, labeling with heavy-atom derivatives and Se-met incorporation, X-ray data collection, 3D-model building and PDB structure deposition.♦ Structural analysis of covalently bound carbohydrate and substrate/product containing enzymes to structurally study the enzymes MOA.♦ I conducted an exhaustive structure guided active-site mutational analysis and elucidated the molecular mechanism of catalysis assisted by enzyme kinetics.

Rehovot, Hamerkaz, Il

Biochemical and structural characterization of an engineered 3‐finger snake toxin♦ Protein engineering of a chimera protein inhibitor for Acetylcholinesterase.♦ Protein purification enzyme activity assay.

Postdoctoral Research Scholar, Structural Biology - Ion Channels

Doctor Of Philosophy (Ph.D.), Biochemistry

M.Sc, Structural Biology