Dennis Ramey Email & Phone Number

Greater Seattle Area

Manage and train a team of 10+ employeesSchedule staffing at festivals and weekly farmers marketsMaintain in depth financial records of income and expenses; prepare and file business tax returnsProvide excellent customer service to clientsCoordinate advertising on social media and the company website

- Lead development and planning of an in-house lentiviral production facility to support clinical trials- Culture research and therapeutic human T-cells in support of pediatric leukemia and neuroblastoma clinical trials- Familiarity with clinical GMP practices- Experience with proper cleanroom etiquette and environmental monitoring techniques- Lead process development experiments to improve current clinical protocols with the objective of improving patient safety and reducing… Show more - Lead development and planning of an in-house lentiviral production facility to support clinical trials- Culture research and therapeutic human T-cells in support of pediatric leukemia and neuroblastoma clinical trials- Familiarity with clinical GMP practices- Experience with proper cleanroom etiquette and environmental monitoring techniques- Lead process development experiments to improve current clinical protocols with the objective of improving patient safety and reducing waste- Author and revise standard operating procedures to document clinical production and reagent preparation- Familiarity with continuous performance improvement and developing a culture of 'lean' practices Show less

Greater Seattle Area

- Generate and optimize a Nuclear Factor of Activated T-cells (NFAT) transcription factor responsive vector for use with various genes of interest- Apply molecular biology techniques for the subcloning and manipulation of plasmid DNA for cancer immunotherapy applications- Culture various mammalian cell lines, including tumor and therapeutic lines- Generate lab stocks of chemically competent bacteria, mammalian and bacterial culturing reagents- Analyze DNA sequencing data for QC… Show more - Generate and optimize a Nuclear Factor of Activated T-cells (NFAT) transcription factor responsive vector for use with various genes of interest- Apply molecular biology techniques for the subcloning and manipulation of plasmid DNA for cancer immunotherapy applications- Culture various mammalian cell lines, including tumor and therapeutic lines- Generate lab stocks of chemically competent bacteria, mammalian and bacterial culturing reagents- Analyze DNA sequencing data for QC of new and existing plasmids- Generate master bank and lab stocks of plasmid DNA- Isolate PBMCs from whole blood using Ficoll separation- Collect and analyze single or multi-color flow analysis data for evaluation of plasmid functionality, transduction efficiencies, and viral titers- Perform routine mycoplasma testing of cultured cell lines- Train and manage a team of 4-6 volunteers in performing basic lab techniques and general lab maintenance- Thoroughly document all experiments using the iLabber electronic lab notebook- Aid in receiving and maintain lab stocks of various reagents and materials- Aid in laboratory equipment maintenance procedures and quality control monitoring Show less

- Instruct teaching assistants and provided in lab support to ensure safe completion of undergraduate chemistry lab experiments- Ensured students and TAs were in agreement with departmental safety requirements regarding laboratory use- Manage a team of 6-8 student helpers to aide in the operation of the Chemistry Undergraduate Stockroom- Created chemical solutions and managed waste produced, including labeling and following proper disposal protocols- Maintained and… Show more - Instruct teaching assistants and provided in lab support to ensure safe completion of undergraduate chemistry lab experiments- Ensured students and TAs were in agreement with departmental safety requirements regarding laboratory use- Manage a team of 6-8 student helpers to aide in the operation of the Chemistry Undergraduate Stockroom- Created chemical solutions and managed waste produced, including labeling and following proper disposal protocols- Maintained and fixed/replaced malfunctioning equipment in a timely manner to ensure seamless completion of laboratories Show less

Nesac/Bio & Castner Lab, Departments Of Chemical And Biomedical Engineering

- Study amino acid composition of cytochrome c- Use surface analysis techniques including XPS and ToF-SIMS, as well as cyclic voltammetry to study and characterize immobilized cytochrome c on carboxyl and amine terminated SAMs- Experienced in SAMs preparation and protein adsorption- Co-authored publication on research findings

Seattle, Wa

- Worked closely with Lab Technicians to ensure a safe learning environment and smooth completion of lab coursework for 2500+ students- Managed stockroom responsibilities and developed novel solutions to time sensitive issues- Provided excellent customer service to undergraduate students

Lynnwood, Washington

- Tutor study abroad students in biology, calculus, chemistry, and physics- Safely transport students for college organized activities in the greater Seattle area

- Provided excellent customer service- Completed sales of merchandise

Certificate, Master Brewer

Bachelor Of Science (Bs), Bioengineering

Minor, Chemistry