Devendra Chauhan, Phd Email & Phone Number

California, United States

Switzerland 🇨🇭 , Ch

• Leading Reagents group in Roche Diagnostics (Roche Sequencing Solutions) with Ph.D. and non-PhD level scientists (Total +12 direct/indirect reports).• Responsible for providing membrane protein complexes, Protein-DNA complexes ssDNA, dsDNA, genomic DNA templates, and all buffer formulations for single-molecule nanopore-based NGS platform development in R&D. • Microbial fermentation, expression of DNA polymerase and membrane proteins.• Process development and scale-up purification of enzymes, membrane proteins, and protein-DNA complexes. • Assay development and analytical characterization of reagents using HPLC, DSF, CE, DLS, ELISA, fluorescence, and colorimetric methods. • Technology transfer, process verification, and development of QC specifications for the DEV & manufacturing.• Development of new QC platforms for analysis of proteins and reagents.• Biocide compatibility studies and raw material QC specifications for IVD reagents.• High throughput reagent stability and formulation studies by DSF.• Lyophilization of protein complexes and reagents.• Preliminary real-time and accelerated stability studies for NGS reagents.• Identification of equipment and resources needed to complete the projects.• Supply chain and inventory management. • Excellent organizational, time management, communication, and multi-tasking skills.• Excellent troubleshooting, data analysis, and presentation skills.

VLP, Vaccine development, Bio-conjugation, Click Chemistry, Construct design, Endotoxin, Fusion proteins, Protein expression, Protein purification, Antibody development, Antibody purification, Antibody fragmentation, protein-protein interactions, Antibody-antigen binding kinetics, Forte-Bio, FPLC, HPLC, UPLC, N-Glycan analysis, Pre-clinical reasearch

Waltham, Ma, Us

• Fermentation, protein purification, formulation and QC analysis of various protein products• Troubleshooting and process development• Fermentation of E.Coli & Pichia pastoris in 12 -30 liters fermenters• Large scale clean room purifications of several high profile enzymes by AKTA pilot/explorer using various chromatographic techniques• QC analysis of enzymes by Radiometric, Florescence, RT-PCR, UV-Abs assays• Working experience with SOP writing, cGPM, 5S, LIMS, SAP, Trackwise, Documentum, Matric systems ISO and USDA regulated environments• Handled multiple projects with overlapping deadlines in a fast paced working environment• Supervision and training of people in multidisciplinary environments

Tempe, Az, Us

● Purification of membrane/soluble proteins by AKTA● Column chromatography.● Characterization of the proteins by protein assays, ulta-centrifugation, chromatography, 2D gel electrophoresis, Immunoblot, dual beam UV-Vis, fluorometry and ICP.● Protein re-folding, crystallization, crystal mounting, X-ray data collection, structure solution. ● Laser absorption spectroscopy, streak camera, Circular dichroism, dynamic light scattering, EPR, electron microscopy, kinetics and MALDI MS.● Optimization of cell growth and protein-purification/-stability/-storage conditions.● Experience in X-ray data collection at synchrotron in various national laboratories.● 3+ years of experience in teaching and supervision of chemistry labs in ASU. ● Excellent interpersonal, communication and presentation skills.● Excellent trouble-shooting and data analysis skills.

Uttarakhand, In

● Removal of toxic metal ions and chemicals from industrial waste.● Development of chemical sensors for the toxic metal ion determination.● CPR certification, Lab safety, Laser safety, OSHA safety training.

Phd, Biochemistry

Ms, Chemistry

Ms, Industrial Chemistry