Rajeshwari Chellappan Email & Phone Number

Rochester, Minnesota, United States

Department Of Pathology - Volker Hall

● In-vivo mouse model of Fungal / Aspergillus Keratitis, COVID associated Pulmonary Aspergillosis andMucromycosis to evaluate new antifungal drug candidates● In-vitro SARS-CoV2 model for evaluation of new antiviral candidates● Corneal, i.v., i.p., s.c. injections in mice, blood collection from tail vein, necropsy and lung, heart, liver &eye collection, sample preparation for downstream assays● Performed transcriptomics experiments to localize SARS-CoV2 and immune markers in mice lung tissue such as RNA-seq with NGS / Next Generation Sequencing● Assisted in genomics experiments including sample preparation, processing, library generation, PCRamplification through high-throughput NGS based automation system (Genexus) for identification ofCOVID-19 variants to develop PCR-based molecular assays at UAB FRL● Set up and maintained Vero, HEK293, CHO and A549 cells for viral culture / viral vector production● Performed ELISA, Western blotting, multicolor flow cytometry of immune cells, PCR, qPCR● Performed IHC and ISH of Human ARDS lung samples for SARS-CoV2 diagnosis confirmation● Performed general lab duties - inventory, cataloging, lab instrument QC, maintenance, calibration, SOPdevelopment, preparation for annual and triennial IACUC/EHS audits, maintaining stock supplies,adherence to budgets and timelines, one-on-one and lab meetings set up and minute documentation● Generated purchase order, placed orders with vendors / suppliers, managed receiving● Recorded temperature, humidity, fridge / freezer / liquid nitrogen tanks for cell storage● Mentored several trainees and graduate students to perform essential molecular biology experiments● Set up several new instrumentations in BSL/ABSL-3 lab and acted as POC for training and scheduling● Maintained 20+ lines of mouse colonies for in-vivo model development● Collaborated in-person with LV Prasad Eye Institute, India & mentored researchers to perform Mucor IHC

Department Of Neurology - Civitan International Research Center

● In-vivo mouse model of PDX-glioblastoma, Parkinson’s, Neuroinflammation, spinal cord injury● Performed stereotaxic brain injections in over 100+ mice● Performed clinical and behavioral assessment of mice using Basso Mouse scale, horizontal ladder test● In-vitro model for evaluation of new small-molecule drugs in inflammation● Brain, i.v., i.m, s.c., i.p. injections in mice, blood collection from cardiac and tail vein, necropsy and braincollection, sample preparation for downstream assays● Performed genomics experiments such as whole genome sequencing to identify SNPs encoding differentinflammatory markers, micro-arrays to find any new genetic signatures in neuroinflammation, RNA-seq,gene knockout/silencing using siRNA● Set up and maintained mammalian cell cultures of primary microglial / astrocytes / mixed glial culture andBV2 / HeLa cell lines to study inflammation and new small molecule inhibitor drugs● Performed ELISA, Western blotting, multicolor flow cytometry / FACS sorting of immune cells, PCR, qPCR● Performed histology experiments such as cryostat sectioning, immunohistochemistry and ISH● Performed cytotoxicity, cytokine release, cell migration, proliferation and lentiviral / AAV transductionassays● Performed confocal and light microscopy● Analyzed data using Prism / FlowJo / ImageJ● Collarborated with multiple departments to facilitate project completion

Department Of Clinical Immunology And Rheumatology - Shelby Building

● In-vivo mouse model of Experimental Autoimmune Encephalitis● Performed clinical and behavioral assessment of mice● Maintained 10 distinct lines of mouse colonies for in-vivo model development● Assisted in performing mice brain, i.p., s.c. injections● Assisted in performing confocal and light microscopy

Coimbatore Area, India

● Rotations in a 1400-bedded hospital including postings in Pathology, Neurology, Surgery● Developed skills in hematopathology, cytology, autopsy and microbiology

Master’S Degree, Public Health - Biostatistics, Magna Cum Laude

Activities and Societies: UAB Hoke Hire Academic Center TEDxUAB UAB Multicultural Council UAB Association of Indian Students UAB.

Bachelor Of Medicine And Bachelor Of Surgery - Mbbs, Medicine, Cum Laude

(US equivalency of Doctor of Medicine (MD) as evaluated by World Education Services, not American Board Certified) Passed USMLE Step 1.