Duc Phan Email & Phone Number

Cambridge, Massachusetts, United States

• Works with a senior scientist on an exploratory project to develop and optimize in vitro cell-based assays that can be translated into in vivo mouse studies• Aseptic isolation, propagation, and cultivation of primary or established mammalian cell lines and tissue cultures• Performance of analytical assays and complex multi-day cell-based functional assays (ELISA, qPCR, flow cytometry, Western Blotting) to support antibody characterization and/ or pharmacology studies• Analysis, interpretation, and presentation of the results to all the relevant groups and team meetings• Maintenance of detailed records of experimental protocols and data in the laboratory notebook in accordance with company policies

• Conducted an electrophysiology project on elucidating mechanisms of actions of Spec 36 specificity on Kir 3.1-containing subunit• Performed TEVC to generate results to produce supporting data for the paper on a role of Spec 36 in the regulation of GIRK (Kir3) heterotetramers channels• Executed site-directed mutagenesis tests to verify critical binding sites and elucidate the drug-ligand binding mechanisms of Spec 36 on homomeric and heteromeric GIRK channels• Participated in weekly lab journal club meetings to discuss and analyze biomedical-related scientific papers to enhance the understanding of the pharmacology and pathophysiology of GPCR (G-protein-coupled receptors) signaling mechanisms in health and diseases• Analyzed data with GraphPad Prism, Excel, and R programming to evaluate statistically the potency, efficacy, and specificity of Spec 36

• Conducted a project about overviewing drugs differentiate between homomeric& heteromeric subunits of GIRK (Kir3) channels• Performed electrophysiology technique (TEVC – two-electrode-voltage clamp) to produce dose-response curves of IVM (Ivermectin), VU0529331, and Spec 59 and assess their selectivity for GIRK 2, GIRK 4, GIRK 1/2, and GIRK 1/4• Executed site-directed mutagenesis tests to verify critical binding sites and elucidated drug-receptor interaction mechanisms of VU05239931 and Spec 59 on GIRK 4• Elucidated mechanisms of actions of small molecules that target GIRK (G protein-gated inwardly rectifying K+) channels as well as examine the dependence of GIRKs activity on phosphatidylinositol 4,5-bisphosphate (PIP2) • Produced a scholarly work that puts a testable hypothesis, background, experimental data, results, and discussion into a literature context

Boston

• Worked closely with a research faculty (mentor) to be trained in electrophysiology method (two-electrode-voltage clamp – TEVC)• Learned to do all other associated lab techniques to conduct TEVC experiment (DNA/RNA making with T7 Transcription Kit, gel electrophoresis, buffer preparation, cell isolations, oocyte injection)• Performed TEVC to screen compounds (from drug library) to identify potent and efficacious small-novel molecules that can activate/ inhibit with complete specificity for GIRK (Kir3) channel• Data analysis using GraphPad Prism, Excel and R program to assess statistically the potency, efficacy, and selectivity of new compounds• Attended weekly personal meetings with Dr. Logothetis to gain insights into the laboratory research background with a focus on GIRK channels and their activity via the master regulator of membrane protein function, phosphatidylinositol bis-phosphate (PIP2)

Boston, Massachusetts, United States

• Served as an essential resource during the welcoming week for newly coming freshmen • Hosted orientation week program and information session to aid newly enrolled undergraduate students and families to locate resources, programs, and platforms to chart their Northeastern path with global research, and experiential opportunities during their transition period to the university• Assisted students in navigating and building networks within the Northeastern global community, and enhanced their “Husky” experience (both academic & social experiences)

Boston, Massachusetts, United States

• Responsible for providing access control at Northeastern University residence halls• Maintained a quiet, well-lit, open space area and monitored surrounding activities to ensure a secure environment as well as reported any suspected irregularities to the supervisor• Serve as the first contact for non-residents/ guests, on behalf of the university, by providing basic campus information, miscellanies/ trivia, and helpful resources

Woburn, Massachusetts, United States

• Planned and performed hands-on laboratory experiments using chromatographic analysis (HPLC) and UV-Vis spectrometer• Conducted dissolution-testing experiment to assess the stability and efficiency of the drug• Contributed and maintained detailed technical documentation (experimental plans, standard operating procedure, laboratory reports)• Prepared buffers, and experimental chemicals (assays, organic solvents, mobile phase), and assisted other chemists as required

Ho Chi Minh City, Vietnam

• Consulted and helped about 20 patients daily understand the prescription and OTC drugs• Learned more about commonly prescribed drugs on the market (e.g., cardiovascular agents, analgesics, and antidiabetic drugs)• Monitored inventory daily selling drugs with low capacity and order replenishment in accordance with the pharmacy market’s plan• Labeled and stocked medicine in storage within the Ministry of Health's compliance

Ho Chi Minh City, Vietnam

• Translated training materials related to company medicinal products, and pathologylectures from English to Vietnamese and vice versa; translated and proofread standardoperation procedures (SOPs) documents• Organized, planned, and hosted product training sessions for the company pharmacists• Attended, took notes, and learned about pharmacology of the drug from educationsession lectured by doctors, pharmacists, and scholars

Master Of Science - Ms, Pharmaceutic And Drug Delivery Systems, 3.8/4.0

Bachelor Of Science - Bs, Pharmaceutical Sciences, 3.6/ 4.0

High School Diploma