Dylan Dufek Email & Phone Number

United States

Carlsbad, California, United States

Designed and carried out various experiments for the isolation of extracellular vesicles via PEG precipitation, microfluidic immunocapture, size exclusion column purification. Performed downstream molecular analysis of extracellular vesicle samples including RNA isolation, cDNA synthesis, RT-PCR detection of mRNA markers, digital PCR detection of mRNA.

Isolated viral RNA from both human collected samples and heat inactivated SARS-CoV-2 viral particles and carried out multiplex RT-qPCR to assess viral loads of each sample.Assisted with the research and development of a microfluidic assay for the capture and release of SARS-CoV-2 viral particles to distinguish between infectious and non-infectious saliva.

Chemically surfaced microfluidic chips using EDC-NHS chemistry with specific DNA oligos and capture antibodies. Chemical surfacing was performed with Hamilton StarLET liquid handlers.Effectively reduced antibody consumption for microfluidic chip surfacing by 30% without any change in downstream performance of antibody capture.Designed and performed various.

Assembly and running of several protein characterization techniques including BCA assays and ECL western blotting to quantify and identify proteins.Assist researchers through the modeling of cancer growth by fission-yeast cultures. Assist with autoclaving, media preparations, buffer preparations, sample acquisition, and stocking and maintenance of tissue.