Dylan Mclaughlin Email & Phone Number

Boston, Ma, Us

Cambridge, Massachusetts, United States

- Utilize molecular cloning (golden gate assembly, restriction-ligation cloning) and other molecular biology techniques (such as PCR, bacterial transformation, and restriction digests) to generate CRISPR, RNAi, and other perturbation libraries for laboratories to use in their genetic screens. - Implement rigorous quality control experiments to ensure the DNA constructs present in the libraries are high quality, uncontaminated, and evenly represented to ensure the DNA would not cause a collaborators screen to fail.- Perform polymerase chain reaction for use in next generation sequencing on genomic DNA extracted and sent to GPP by our various collaborators. - Analyze protocols and experimental data regularly to streamline standard operating procedures to minimize future errors.- Maintain and catalogue a large collection of plasmids and glycerol stocks for use within GPP and for use by collaborators.- Implement best laboratory notebook practices to record experimental planning and results.- Cooperate with a diverse group of research associates, research scientists, laboratory technicians, laboratory operations coordinators, and project managers.

Massachusetts, United States

- Facilitated biochemical and molecular biology experiments such as immunoblots, restriction digests, affinity chromatography, and kinetic assays.- Collaborated with fellow teachings assistants, lab staff, and professors to divide tasks and expedite procedures. - Guided students on forming explanatory hypothesis based on observation and designing their own experiments

University Of Massachusetts, Amherst

- Conducted research under the guidance of our Principle Investigator and Post-Doctoral Researcher.- Created CRISPR-Cas9 containing plasmids and transformed them into A. thaliana plants to generate null mutants. - Extracted genomic DNA and performed PCR experiments to identify and genotype mutant plants.- Evaluated extracted proteins from mutant plants to determine protein expression levels of mutant plants. - Compiled and analyzed data culminating in a senior honors thesis investigating small heat shock proteins and their role in the heat stress response of the model organism Arabidopsis thaliana. - Taught other undergraduates and technicians biochemical and molecular biology techniques and protocols used by the lab.

Watertown, Massachusetts, United States

- Cultured mammalian cancer cell lines for use in pharmacological experiments including MOA analysis of drug candidates in ELISA and western blot techniques. - Fostered best practices for experimental design by incorporating appropriate controls and internal standards. - Maintained sterile techniques in biosafety cabinets for the purpose of working with healthy donor whole blood samples. - Cooperated with other scientists and interns to complete experiments according to a specific timeline.

Waltham, Massachusetts, United States

- Enforced pool rules and ensured the safety of pool attendants.- Tested pool water regularly to check the levels of various chemicals.- Documented the results for the records of the pool and the city of Waltham, Massachusetts.

Waltham, Massachusetts, United States

- Addressed customer questions and concerns while answering phones- Organized orders between the front of house and the kitchen. - Coordinated with other staff members to ensure the success and efficiency of the restaurant.- Trained several employees with the skills they needed to succeed in similar positions.

Bachelor Of Science - Bs, Biochemistry And Molecular Biology

Activities and Societies: Member of the Commonwealth Honors College, Peer Mentor in the Biochemistry Department, Undergraduate Teaching.

Education record