Isaac García Reyes Email & Phone Number

United States

Cambridge, Massachusetts, United States

Monterrey, Nuevo León, Mexico

• Retrieving reported information regarding 3D-bioprinting techniques to enhance cell alignment and bioink formulations.
• Patent searching through different databases such as WIPO, Scopus, Lens, etc.

Saudi Arabia

• Stem cell and disease lab. PI: Antonio Adamo, Ph.D.
• Cultured, maintained, and differentiated iPSCs and hESCs into β-cells in both 3D and 2D models.
• Standardized Fluorescence-activated Single Cells (FACS) throughout the β-cell differentiation stages.
• Characterized protein and gene expression employing several molecular biology techniques: western blot, RT-qPCR (TaqMan), and immunostaining.
• Performed ELISA assay to detect and quantify insulin release from iPSCs-derived β-cells.

Boston, Massachusetts, United States

• Biomedical Engineering Lab. PI: Su Ryon Shin, Ph.D.
• Cultured, maintained, and differentiated iPSCs into muscle skeletal cells.
• Employed stand-alone 3D bioprinting techniques to create tissue-engineered cell-laden constructs usingsmart biomaterials.
• Performed immunostaining and in vitro characterizations on 3D cell-laden constructs.
• Harvested cell-released exosome to deliver genetic material for cell reprogramming purposes.
• Regenerated cardiovascular tissue via the direct reprogramming of mouse fibroblast.

• Alvarez&Trujillo Lab:Project: Anticancer Drug Evaluation in MCF7 Spheroids Cultured in a 3D-Printed Miniaturized Continuous Stirred Tank Reactor (mCSTR)
• Cultured cancer cells (MCF7) to render spheroids in ULA well-plates for further anti-cancer drug assessment.
• Extracted and quantified nucleic acids.
• Designed and analyzed Quantitative Reverse Transcription PCR (RT-qPCR) and Endpoint PCR.

Monterrey, Nuevo León, Mexico

• Mammalian cell culture
• Immunostaining for fluorescence analysis
• Extraction and quantification of nucleic acids for posterior amplification by RT-qPCR
• Experimental design for microfluidic device

• Polyhydroxyalkanoate (PHA) production via microalgae fermentation using food wastes as a carbon source.
• Inoculated and cultivated Botryococcus braunii and Spirulina maxima (cyanobacteria).
• Analyzed and measured cyanobacteria growth using optical density and their dry weight.

Biotechnology Engeneering, Biotechnology, Bachelor Of Science

Visiting Student, Bioengineering

Activities and Societies: Occasional Student at Microbial Engineering and Bioprocess Design and Stem Cell & Molecular Medicine courses.

Exchange Research Program, Biomedical/Medical Engineering