Isaac García Reyes Email & Phone Number

United States

Cambridge, Massachusetts, United States

Monterrey, Nuevo León, Mexico

◦ Retrieving reported information regarding 3D-bioprinting techniques to enhance cell alignment and bioink formulations.◦ Patent searching through different databases such as WIPO, Scopus, Lens, etc.

Saudi Arabia

Stem cell and disease lab. PI: Antonio Adamo, Ph.D.◦ Cultured, maintained, and differentiated iPSCs and hESCs into β-cells in both 3D and 2D models.◦ Standardized Fluorescence-activated Single Cells (FACS) throughout the β-cell differentiation stages.◦ Characterized protein and gene expression employing several molecular biology techniques: western blot, RT-qPCR (TaqMan), and immunostaining.◦ Performed ELISA assay to detect and quantify insulin release from iPSCs-derived β-cells.

Boston, Massachusetts, United States

Biomedical Engineering Lab. PI: Su Ryon Shin, Ph.D.◦ Cultured, maintained, and differentiated iPSCs into muscle skeletal cells.◦ Employed stand-alone 3D bioprinting techniques to create tissue-engineered cell-laden constructs usingsmart biomaterials.◦ Performed immunostaining and in vitro characterizations on 3D cell-laden constructs.◦ Harvested cell-released exosome to deliver genetic material for cell reprogramming purposes. ◦ Regenerated cardiovascular tissue via the direct reprogramming of mouse fibroblast.◦ Synthesized and characterized mechanical properties of biomaterials.

Alvarez&Trujillo Lab:Project: Anticancer Drug Evaluation in MCF7 Spheroids Cultured in a 3D-Printed Miniaturized Continuous Stirred Tank Reactor (mCSTR)◦ Cultured cancer cells (MCF7) to render spheroids in ULA well-plates for further anti-cancer drug assessment. ◦ Extracted and quantified nucleic acids.◦ Designed and analyzed Quantitative Reverse Transcription PCR (RT-qPCR) and Endpoint PCR.

Monterrey, Nuevo León, Mexico

◦ Mammalian cell culture◦ Immunostaining for fluorescence analysis◦ Extraction and quantification of nucleic acids for posterior amplification by RT-qPCR◦ Experimental design for microfluidic device

Polyhydroxyalkanoate (PHA) production via microalgae fermentation using food wastes as a carbon source.◦ Inoculated and cultivated Botryococcus braunii and Spirulina maxima (cyanobacteria).◦ Analyzed and measured cyanobacteria growth using optical density and their dry weight.

Biotechnology Engeneering, Biotechnology, Bachelor Of Science

Visiting Student, Bioengineering

Activities and Societies: Occasional Student at Microbial Engineering and Bioprocess Design and Stem Cell & Molecular Medicine courses.

Exchange Research Program, Biomedical/Medical Engineering