Earl Kim Email & Phone Number

San Diego, California, United States

• Development of therapeutic and reagent monoclonal antibodies using Hybridoma and Phage Display technology.
• Viral transfection for generating transient or stable ox cell lines. Qualified engineered cell lines for screening portfolios.
• Certified for Laboratory Animal Handling (immunizations, sacrifice, organ harvest, etc), trained vivarium colleagues with experimental immunization techniques.
• Responsible for procurement of new laboratory automation equipment; programming and validating new methods. IQ/OQ/PQ
• Provided support for flow core and insight for developing flow-based functional assays.
• Target validation and provided insight towards toxicity/efficacy studies.

South San Francisco

South San Francisco, CA

• Development of therapeutic and reagent monoclonal antibodies using hybridoma technology
• Design of immunization and screening strategies for complex targets on endogenous and over-expressing cell lines via flow
• Optimization of mouse immunization strategies and immune response checks
• Execution and optimization of robotic high throughput screening for selection of drug candidates
• Responsible for transitioning manual laboratory processes to automated platforms & streamlining.
• Modernized and updated new robotic platforms for increased throughput and improved assay output

South San Francisco, California, United States

South San Francisco, California, United States

South San Francisco, CA

• Increased efficiency for hybridoma/antibody production by incorporating various fully-automated liquid handling processes to cell culture maintenance and HT screening
• Developed various aggressive ELISA protocols used for selection and screening hybridoma supernatants.
• Cell fusion techniques for generating hybridoma cells (primarily electrofusion)
• Validated assays in automation studies and recognized for developing novel screening technology.
• Lead small molecule candidate characterization and tool study for auto-immune disorders.
• Immune-based and cell-based assay development and optimization [ELISA / FACS / Alpha / HTRF / FRET]

South San Francisco, California, United States

South San Francisco, CA

• Specialty DNA subcloning and troubleshoot projects
• PCR amplification, colony PCR
• Vector construction/modification
• Cloning by homologous recombination
• Site-directed mutagenesis and deletions
• DNA sequence analysis and primer design

Vacaville, CA

• Support service for process development labs (MSATS) and QC labs
• Assembled and maintained bioreactors and prototyped filter assemblies
• Maintained sanitized and sterilized laboratory glassware
• On-call service for urgent assembly requests.
• Volunteered responsibility for weekly inventory of laboratory consumables
• Reduced cell-culture spinner and bioreactor assembly delays

• Prepared tissue samples for digestion and heavy element analysis
• Data-mining and client catalogs/ascensions management
• Analysis by ICP and AA Spectrophotometers
• Basic laboratory maintenance
• Streamlined data-to-client turnover from three days, to one.
• Sterilized test tubes, upkeep of sterile work environment

• Organized team roster and appointed competitive seeding
• Managed team and club practice scheduling every academic quarter
• Representative for monthly campus sport club organizational meetings
• Updated competition schedules
• Supervised varsity team practices
• Lead team to two consecutive conference championships

UC Davis VMTH

• Diversified extraction/detection protocol for detection of BPA-contaminants in cat food
• Troubleshooting and Calibration of HPLC equipment (Agilent 1090LC and 1100LC)
• Various fractionation and filtration techniques
• Extensive familiarity with Agilent Chemstation and Cambridgesoft Chemdraw programs