• Development of therapeutic and reagent monoclonal antibodies using Hybridoma and Phage Display technology.• Viral transfection for generating transient or stable ox cell lines. Qualified engineered cell lines for screening portfolios.• Certified for Laboratory Animal Handling (immunizations, sacrifice, organ harvest, etc), trained vivarium colleagues with experimental immunization techniques.• Responsible for procurement of new laboratory automation equipment; programming and validating new methods. IQ/OQ/PQ• Provided support for flow core and insight for developing flow-based functional assays.• Target validation and provided insight towards toxicity/efficacy studies.• Small/Medium-scale antibody purification (gravity/tip and GE-AKTA) and basic characterization.• Trained and supervised junior staff members with HT automation, robotics usage, and troubleshooting.• Liaison and point of contact for process implementation and qualifying new workflow modules.• Assisted senior staff with delegating process improvement studies and scheduling new/renewed antibody program studies

• Development of therapeutic and reagent monoclonal antibodies using hybridoma technology• Design of immunization and screening strategies for complex targets on endogenous and over-expressing cell lines via flow• Optimization of mouse immunization strategies and immune response checks• Execution and optimization of robotic high throughput screening for selection of drug candidates• Responsible for transitioning manual laboratory processes to automated platforms & streamlining.• Modernized and updated new robotic platforms for increased throughput and improved assay output• Target validation and provided insight towards toxicity/efficacy studies• Supported discussion regarding antigen source and functional screening assays• Single-cell discrimination and sorting via flow cytometry, multicolor flow• Responsible for implementing and optimizing flow titration assays for therapeutic candidates against multi-pass targets.

• Increased efficiency for hybridoma/antibody production by incorporating various fully-automated liquid handling processes to cell culture maintenance and HT screening• Developed various aggressive ELISA protocols used for selection and screening hybridoma supernatants. • Cell fusion techniques for generating hybridoma cells (primarily electrofusion)• Validated assays in automation studies and recognized for developing novel screening technology.• Lead small molecule candidate characterization and tool study for auto-immune disorders.• Immune-based and cell-based assay development and optimization [ELISA / FACS / Alpha / HTRF / FRET]• Lead molecule de-orphanization through HT-Screening and ECD-Fc characterization• Specialty DNA subcloning and vector manipulation for in vivo immunization • Aseptic and sterile technique for handling tissue/cell culture (BSL2)• FAB molecule manipulation and characterization• Characterization and Identification of new lead candidate molecules• Fully-trained and certified for laboratory animal handling

• Specialty DNA subcloning and troubleshoot projects• PCR amplification, colony PCR• Vector construction/modification• Cloning by homologous recombination• Site-directed mutagenesis and deletions• DNA sequence analysis and primer design• Plasmid DNA transformation reactions (using electroporation and chemical transformation methods)• Knowledge of high-throughput cloning and screening• Plasmid DNA miniprep, maxiprep, and megaprep• Plasmid DNA characterization (restriction mapping and restriction digests)

• Support service for process development labs (MSATS) and QC labs• Assembled and maintained bioreactors and prototyped filter assemblies• Maintained sanitized and sterilized laboratory glassware• On-call service for urgent assembly requests.• Volunteered responsibility for weekly inventory of laboratory consumables• Reduced cell-culture spinner and bioreactor assembly delays• Used and serviced autoclaves daily.• GLP, cGMP, ISO, and FDA compliant.

• Prepared tissue samples for digestion and heavy element analysis• Data-mining and client catalogs/ascensions management• Analysis by ICP and AA Spectrophotometers• Basic laboratory maintenance• Streamlined data-to-client turnover from three days, to one.• Sterilized test tubes, upkeep of sterile work environment• ICP and AA calibration• GLP compliant

• Organized team roster and appointed competitive seeding• Managed team and club practice scheduling every academic quarter• Representative for monthly campus sport club organizational meetings• Updated competition schedules • Supervised varsity team practices• Lead team to two consecutive conference championships• Assistant coach during competitions/tournaments • 2008 Outstanding Leadership Award

• Diversified extraction/detection protocol for detection of BPA-contaminants in cat food• Troubleshooting and Calibration of HPLC equipment (Agilent 1090LC and 1100LC)• Various fractionation and filtration techniques• Extensive familiarity with Agilent Chemstation and Cambridgesoft Chemdraw programs