Explore Tyrosine Phosphorylation-related Signal Pathway of CD28 and other immunoreceptors/ligands by MS-based proteomics

 Extraction, analysis and purification of key compounds (secondary metabolites) in Ginkgo biloba leaf extract and side-stream wastes from Cara Partners Ltd.(Process Development, Liquid extraction/supercritical fluid extraction, Sephadex and silica gel column chromatography, HPLC or Agilent Q TOF LC-MS, light absorption-based antioxidant assays, ANOVA)

Postgraduate student Nucleic Acid Research Group, School of Chemistry, University of Southampton  Synthesis of one cyclooctyne compound and tests of DNA copper-free “click” ligation (5-step organic synthesis of a cyclooctyne NHS ester, 1H/19F/13C 1D and 2D-NMR, Mass Spec., Biotage® automated flash chromatography, DNA chemical labelling, PAGE, LC-MS, UV-melting spectroscopy) Established the synthesis procedures of modified nucleoside triphosphates for the research group (both liquid and solid-phase triphosphorylation, 1H/31P/13C 1D and 2D-NMR, Bruker micrOTOF LC-MS) Solid-phase synthesis and purification of modified DNA and RNA oligonucleotides and their Click ligation products (automatic solid-phase synthesis, preparative HPLC, PAGE, capillary electrophoresis, Bruker micrOTOF LC-MS) DNA polymerase, RNA polymerase, T4 RNA ligase and reverse transcriptase reaction evaluations (PCR, PAGE, Agarose gel electrophoresis, Bruker micrOTOF LC-MS, DNA and RNA enzymatic labelling)

 Leading the gene mapping program of BIO (BIG ORGAN in Lotus. japonicus) Participated in the gene mapping program of NL1 (NECK LEAF 1 in rice)(Large-scale DNA extraction, PCR of SNP markers, PAGE, Agarose gel electrophoresis)