Antibody Purification and CharacterizationFPLC, SEC, IEC, IEX, Affinity ChromatographyHPLC, SECMSCEAutomation

Co-crystallized protein-ligand complexes for 5 different targets for structural determination by X-ray crystallographySoaked ligands into apo crystals for structure determinationCrystallized antibodies, ligases, proteases, and chaperones to support lead discovery Screened a compound library by soaking individual compounds into apo crystals and collecting 2200 data setsSet up a high-throughput co-crystallization screen of 24,000 compounds with a target… Show more Co-crystallized protein-ligand complexes for 5 different targets for structural determination by X-ray crystallographySoaked ligands into apo crystals for structure determinationCrystallized antibodies, ligases, proteases, and chaperones to support lead discovery Screened a compound library by soaking individual compounds into apo crystals and collecting 2200 data setsSet up a high-throughput co-crystallization screen of 24,000 compounds with a target proteaseScreened crystals, evaluated data quality, and set up data collectionsRan automation instruments including Crystalmation, mosquito and ACTOR. Designed and prepared crystallization screens Used only micrograms of proteins to successfully determine conditions with Fluidigm’s fluidic chip technology (FID)Evaluated problems on all automated systems and coordinated repairs with vendorsProduced crystals of a novel previously unsolved multidomain protein which resulted in a structure solved by MAD phasingCommunicated with protein purification group to help optimize protocols Show less

Expressed and purified nitrogenase proteins by FPLC for structure and function analysisCharacterized proteins with SDS PAGE and activity assaysSet up and monitored crystallization trials (FID capillary tubes) for analysis by the ALS Designed and prepared crystallization reagents to optimize crystals

Chemistry and Biochemistry Dept, John Peters LabExpressed, purified and characterized 3 anaerobic metalloproteins Routinely used AKTA prime system to purify protein by FPLC, SEC, IECGrew anaerobic crystals for collection on beamlines and in house sourceAssisted with SLAC data collection Cultured bacteria and archaea for metabolic studies Thermal Biology Institute, Tim McDermott Lab Collected microbes from thermal springs in Yellowstone National Park to… Show more Chemistry and Biochemistry Dept, John Peters LabExpressed, purified and characterized 3 anaerobic metalloproteins Routinely used AKTA prime system to purify protein by FPLC, SEC, IECGrew anaerobic crystals for collection on beamlines and in house sourceAssisted with SLAC data collection Cultured bacteria and archaea for metabolic studies Thermal Biology Institute, Tim McDermott Lab Collected microbes from thermal springs in Yellowstone National Park to assemble a culture collectionExtracted DNA from culture collection to be sequenced for species identificationPerformed study on bioremediation of contaminated soil with culture collectionChemistry and Biochemistry Dept, Martin Lawrence LabHelped characterize proteins for structural biology labResponsible for sanitizing and cleaning laboratory equipment Show less