Eric Buxton Email & Phone Number

Greater San Diego Area

10550 N Torrey Pines Rd La Jolla, CA 92037

Worked as part of the Transport PDB structure determination pipeline in the Chang Lab over expressing, purifying, and crystallizing various human/mammalian/eukaryotic membrane transporter proteins such as P-glycoprotein (Pgp), SLC2A1, SLC2A5, MPDU1, SLC35F1, ABCB6, ABCG5G8, and several others. Successful at this point in getting crystals for SLC2A1, MPDU1.

Various band photography projects.

6313 Nancy Ridge Drive, San Diego, California 92121

Worked as a 1st year Steamfitter Apprentice for A.O. Reed and Bingham Heating and Air.

University Of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093

Worked for UCSD Temporary Services to aid in setting up labs for students in the Division of Biology. Made stock solutions and buffers. Repaired some equipment. Inventoried supplies. Knowledge of UC and EH&S laboratory safety procedures.

10550 N Torrey Pines Rd La Jolla, CA 92037

Worked on an affinity maturation project in the Zwick lab to enhance the binding and neutralization of an anti HIV gp41 antibody via mutagenesis.Cloned several libraries with mutations in the variable antibody CDR region as well as mutations made by error prone PCR. Purified several mutant (Fab) antibodies over protein columns.Ran several ELISAs on crude.

University Of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093

Worked for UCSD Temporary services as a general laborer at the Central Utilities Plant on the UCSD campus.

La Jolla, CA

Worked individually on several projects wherever molecular biology techniques were needed.Cloned several recombinant human antibodies to hCD40, hCD134 (OX40), mLight and the M2 influenza antigen and expressed them in mammalian cells. The hCD40 and M2 influenza projects eventually reached completion and were transferred to Kirin Pharmaceuticals for further.

La Jolla, CA

Worked individually on a project to clone various T-cell signaling genes into a retroviral expression vector and to generate and clone dominant mutants of the wild types

Carlsbad, CA 92008

Designed and ran assays and experiments. Engaged in problem solving and troubleshooting in support of clinical and pre-clinical studies for the development of immune based and gene therapies. Worked in team groups as well as individually on various projects. Contributed to the engineering of a gene delivery system for in vivo therapeutic treatment of HBV.

Worked as a Field Artillery Crewman in the United States Marine Corps Reserves. Active during Operation Desert Storm in 1991.