Erik Rogers Email & Phone Number

4610 S 44Th Place - Phoenix,Az 85040

The IHC Quality Control Technologist will utilize the knowledge necessary to assist pathology professionals with quality control activities to support Immunohistochemistry (IHC), specimen processing, embedding, slide preparation, microtomy, and other technical procedures performed in the laboratory. - Handles receipt, inspection and disposition of incoming reagents. Utilizes functional quality control testing in disposition of incoming critical reagents.- Perform high complexity testing and validation via IHC staining and western blot analysis, utilizing manual and automatic systems.- Successfully executes lab protocols and SOPs.- Prepares antibodies and other solutions for IHC staining.- SOP directed documentation in lab logs and the lab information system (LIS).- Maintain lab equipment and notifies leadership if repairs are needed.- Identify problems that may adversely affect test performance or reporting of tests results and take appropriate action- Assist as needed to perform other related duties and special projects to support the lab, as required.

Http://Rawls.Faculty.Asu.Edu

PI - Dr. Alan RawlsProject - characterizing the role of the transcription factor, Mohawk, in regulating acute and chronic inflammation. Responsibilities and Skills -*Educating volunteer undergrads on the relevance and necessity of the array of techniques employed in the lab, whether by myself, other grad students, or those delegated to my bright eyed, generous undergraduate assistants. *Maintain lab equipment - ensure safe, efficient lab environment *TA duties - guide students through lab experiments and lead related discussions 2 to 3 days/week - grade all relevant assignments and exams in timely manner*Regular presentation and discussion of project data in lab meetings *Publicly present project generated data, analysis and trajectory every year in colloquium *Maintain detailed record of experimental design, execution and outcomes *Variety of differential staining procedures - both paraffin-embedded and frozen tissue - comparing expression patterns of several pro-/anti-inflammatory markers in animals from three novel transgenic murine lines generated in house for the project*Aseptic cell culture - including isolation and propagation of primary myogenic and satellite cell lines *Subsequent comparisons of expression patterns of same pro-/anti-inflammatory markers via qRT-PCR, immunostaining and FACS *Make use of core facilities available to me when needed to further my research goals-FACS - BD FACS calibur cell sorter and analyzer - single 488nm laser cell analyzer-FACS - FACS aria cell sorter - three air-cooled lasers (488nm, 633nm and 407nm)-qRT-PCR - ABI7900HT thermocycler 384 well plate-RNA quantificaion - Agilent 2100 Bioanalyzer 96 well plate and/or Nano-drop absorbance detection -Fluorescence microscopy - Leica TCS SP2 AOBS Laser Scanning Confocal microscope -Plane-polarized light microscopy - Nikon Elipse TE300 inverted microscope.

13208 E. Shea Blvd., Suite 100 - Scottsdale, Az 85259

8 week paid internship at Translational Genomics Research InstituteMentors and PI's: Dr. Kimberly Bussey and Dr. Mike DemeureResponsibilities and Skills:Culture and maintain two different ACC (Adreno-cortical carcinoma) cell lines (H295 and SW-13). Passage, count and calculate necessary number of cells needed to conduct drug dose response assays for both cell lines in the presence of paclitaxel and mitotane. Analyze results of these DDR experiments and present data at weekly lab meetings. Once again had the pleasure of being the first intern to give a 20 minute talk about my project in the ACC lab but as a returning intern and idealistic grad student. Produce a poster detailing my project at the annual Helios Scholars retreat. Maintain detailed record of experimental design, data collection and analyses and any information possibly pertinent to the research goals of my projects and the lab as a whole.Aseptic cell culture techniques ensuring consistent, reproducible results. Transformation of E. coli cells with PCR cloned SIRT1 gene. Subsequently utilized our Flag-tagged SIRT1 sequence to transform both our ACC cell lines. Conduct DDR experiments with both cell lines over-expressing SIRT1 to determine any synergistic relationship with mitotane or paclitaxel. Turned out to be a possible prognosticator of efficacy of mitotane treatment but no synergy or increased stopping power as a clinical combination. Learned importance of generating sufficient data to determine whether a given strategy is worth pursuing in the context of combating ACC - better understanding of the paradigm of high throughput clinical research. Maintaining an accurate accounting of every aspect of my research activities in my trusty lab notebook(s).

Tempe, Az

Arizona State University - School of Life Sciences Undergraduate Research (SOLUR) ProgramAcademic year appointment as a SOLUR FellowMentor and PI: Dr. Jeanne Wilson-RawlsResponsibilities and SkillsTissue collection/banking and processing for histological/histomorphometric analyses. Assist graduate students with various other tasks as needed (i.e., ordering supplies, making necessary reagents, etc.). Produce a poster detailing my project for the annual SOLUR symposium. Give a 20 minute talk detailing my project at the annual SOLUR symposium. Maintain detailed record of experimental design, data collection and analyses and any information possibly pertinent to the research goals of my projects and the lab as a whole.Histology - built on existing repertoire to include extensive work with frozen tissues. Collect and process samples for verification of genotype via PCR. Animal husbandry and handling. Maintaining an accurate accounting of every aspect of my research activities in my trusty lab notebook(s).

445 N. Fifth Street - Phoenix, Az 85004

8 week paid internship at Translational Genomics Research InstituteMentors and PI's: Dr. Kimberly Bussey and Dr. Mike DemeureResponsibilities and Skills:Culture and maintain two different ACC (Adreno-cortical carcinoma) cell lines (H295 and SW-13). Passage, count and calculate necessary number of cells needed to conduct drug dose response assays for both cell lines in the presence of paclitaxel and mitotane. Analyze results of these DDR experiments and present data at weekly lab meetings. Had the pleasure of being the first intern to give a 20 minute talk about my week old project in the ACC lab. Produce a poster detailing my project at the annual Helios Scholars retreat. Maintain detailed record of experimental design, data collection and analyses and any information possibly pertinent to the research goals of my projects and the lab as a whole.Aseptic cell culture techniques ensuring consistent, reproducible results. Transformation of E. coli cells using PCR cloned GOI for future over-expression experiments with both ACC cell lines. Learned importance of generating sufficient data to determine whether a given strategy is worth pursuing in the context of combating ACC - better understanding of the paradigm of high throughput clinical research. Maintaining an accurate accounting of every aspect of my research activities in my trusty lab notebook(s).

Tempe, Az

Arizona State University - School of Life Sciences Undergraduate Research (SOLUR) ProgramAcademic year appointment as a SOLUR ResearcherMentor and PI: Dr. Jeanne Wilson-RawlsResponsibilities and SkillsTissue collection/banking and processing for histological/histomorphometric analyses. Assist graduate students with various other tasks as needed (i.e., ordering supplies, making necessary reagents, etc.). Produce a poster detailing my project for the annual SOLUR symposium. Maintain detailed record of experimental design, data collection and analyses and any information possibly pertinent to the research goals of my projects and the lab as a whole.Histology - became comfortable with all aspects of paraffin embedded tissues. Collect and process samples for verification of genotype via PCR. Animal husbandry and handling. Maintaining an accurate accounting of every aspect of my research activities in my trusty lab notebook(s).

Master Of Science (Ms), Cell/Cellular And Molecular Biology

BS in Molecular Bioscience & Biotechnology SOLUR apprentice, researcher and fellow appointments Helios Scholar intern at Translational.

Bachelor’S Degree, Molecular Bioscience And Biotechnology, 3.5 Gpa

Activities and Societies: Helios Scholar internships in Summers of 2009 and 2010 School of Life Sciences Undergraduate Research (SOLUR).