Fabio Pezzano Email & Phone Number

Position in Prof. Verena Ruprecht lab.- Organized and managed lab operations, including procurement of supplies and communication with the principal investigator.- Provided molecular cloning services for the entire lab, ensuring timely and accurate completion of projects.- Trained and supervised PhD students and post-docs, fostering a collaborative and productive research environment.- Continued to support ongoing research projects, including those focused on nuclear shape and… Show more Position in Prof. Verena Ruprecht lab.- Organized and managed lab operations, including procurement of supplies and communication with the principal investigator.- Provided molecular cloning services for the entire lab, ensuring timely and accurate completion of projects.- Trained and supervised PhD students and post-docs, fostering a collaborative and productive research environment.- Continued to support ongoing research projects, including those focused on nuclear shape and mechanobiology in cancer cells. Show less

Barcellona, Catalogna, Spagna

Position in Prof Verena Ruprecht lab.- Supported international project PLAST_CELL by leveraging expertise in 3D micro-confinement, imaging, and cell culture.- Continued research on the role of nuclear shape in migratory cells using zebrafish models.- Participated in the study of nuclear metabolism and mechanobiology in cancer cells.- Provided technical support and mentorship to researchers and students within the lab.

Barcellona, Spagna

Position in Prof Verena Ruprecht lab.- Utilized zebrafish as a model system to study cellular migration in 3D environments.- Employed advanced microscopy and 3D micro-confinement techniques to identify the nucleus as a mechanosensing element capable of detecting the 3D environment and initiating amoeboid switch and migration.- Extended findings to mammalian systems by working with mouse embryonic stem cells (mESCs) and mammalian cells.- Focused on the study of nuclear shape in… Show more Position in Prof Verena Ruprecht lab.- Utilized zebrafish as a model system to study cellular migration in 3D environments.- Employed advanced microscopy and 3D micro-confinement techniques to identify the nucleus as a mechanosensing element capable of detecting the 3D environment and initiating amoeboid switch and migration.- Extended findings to mammalian systems by working with mouse embryonic stem cells (mESCs) and mammalian cells.- Focused on the study of nuclear shape in migratory cells within 3D environments.- Established a collaboration for xenotransplantation of cancer cells in zebrafish larvae, enhancing the translational impact of the research. Show less

Dipartimento Di Medicina Molecolare E Biotecnologie Mediche, Napoli, Italia

Position in Prof Tommaso Russo lab, under the supervision of Prof. Fabiana Passaro.- Continued the MSc project to finalize the investigation into the crosstalk between the Hippo pathway effector Yap1 and Klf5 protein in mouse embryonic stem cells.- Initiated a new project focused on the direct differentiation of stem cells into cardiac muscle cells.- Designed and performed experiments to optimize differentiation protocols, contributing to the development of effective strategies for… Show more Position in Prof Tommaso Russo lab, under the supervision of Prof. Fabiana Passaro.- Continued the MSc project to finalize the investigation into the crosstalk between the Hippo pathway effector Yap1 and Klf5 protein in mouse embryonic stem cells.- Initiated a new project focused on the direct differentiation of stem cells into cardiac muscle cells.- Designed and performed experiments to optimize differentiation protocols, contributing to the development of effective strategies for cardiac muscle regeneration.- Collaborated closely with senior researchers and supervisors to troubleshoot experimental challenges and refine methodologies. Show less

Dipartimento Di Medicina Molecolare E Biotecnologie Mediche, Napoli, Italia

Position in Prof Tommaso Russo lab, under the supervision of Prof. Fabiana Passaro.- Investigated the crosstalk between the Hippo pathway effector Yap1 and Klf5 protein in mouse embryonic stem cells.- Characterized the expression profiles of Yap1 in differentiated and undifferentiated stem cells, enhancing the understanding of stem cell biology and differentiation processes.- Demonstrated direct interaction between Yap1 and Klf5 in specific subpopulations of stem cells, revealing a… Show more Position in Prof Tommaso Russo lab, under the supervision of Prof. Fabiana Passaro.- Investigated the crosstalk between the Hippo pathway effector Yap1 and Klf5 protein in mouse embryonic stem cells.- Characterized the expression profiles of Yap1 in differentiated and undifferentiated stem cells, enhancing the understanding of stem cell biology and differentiation processes.- Demonstrated direct interaction between Yap1 and Klf5 in specific subpopulations of stem cells, revealing a protective mechanism for Klf5 against proteasome-dependent degradation.- Utilized a range of molecular biology techniques to support findings, including immunoprecipitation, qPCR and protein-protein interaction assays. Show less

Scuola Di Medicina E Chirurgia, Napoli, Italia

I worked as student tutor for medical student. I taught Medical Physics for eight months

Dipartimento Di Medicina Molecolare E Biotecnologie Mediche, Napoli, Italia

Position in Prof. Gabriella Esposito lab.- Conducted Chromatin Immunoprecipitation (ChIP) and quantitative PCR (qPCR) to identify promoter regions influenced by the silencing of the oncogenic MLL-AF4 complex in leukemia.- Assisted in the setup and execution of diagnostic PCR protocols for the identification of Bartter syndrome, contributing to the development of diagnostic tools for this genetic disorder.- Collaborated with team members to analyze and interpret experimental data… Show more Position in Prof. Gabriella Esposito lab.- Conducted Chromatin Immunoprecipitation (ChIP) and quantitative PCR (qPCR) to identify promoter regions influenced by the silencing of the oncogenic MLL-AF4 complex in leukemia.- Assisted in the setup and execution of diagnostic PCR protocols for the identification of Bartter syndrome, contributing to the development of diagnostic tools for this genetic disorder.- Collaborated with team members to analyze and interpret experimental data, contributing to a deeper understanding of the role of 14-3-3 proteins in leukemia. Show less

Doctor Of Philosophy - Phd, Phd In Biomedicine

Thesis: Mechanical control of nuclear plasticity and mechanotransduction in cell migration Supervisor: Dr. Verena Ruprecht

Laurea Magistrale, Biotecnologie Mediche, 110 Cum Laude/110

Master degree in Medical Biotechnologies summa cum laude. Thesis: The Hippo pathway effector Yap1 is involved in Klf5 protein.

Laurea Triennale, Biotecnologie Per La Salute, 110 Cum Laude/110

Bachelor degree in Health Biotechnologies summa cum laude. Thesis (in italian): 14-3-3 theta influenza l'attività oncogenica della.

Diploma Di Scuola Superiore, Liceo Scientifico-Tecnologico, 100 Cum Laude/100