Fadi Azar Email & Phone Number

Strasbourg, Grand Est, Fr

Région De Strasbourg, France

My work consists in developing, optimizing and performing in vitro and in vivo testing in order to contribute in advancing Transgene projects. These projects's aim is to develop innovative immunotherapies to treat solid cancers, by immodulating the tumor microenvironment in favor on antitumor responses.- In vitro skills :Plasmid construction et virus generationPerforming in vitro testing in order to characterize new candidate products of Transgene✔ Mastered cell culture of different tumor lines✔ Performed tests evaluating the oncolytic activity of oncolytic viruses in different tumor lines✔ Performed tests evaluating the replication of oncolytic viruses in different tumor lines✔ Performed viral titrations to identify the presence or absence, and to quantify the virus possibly present in murine tumors✔ Realized extractions of viral DNA for quantification by quantitative Polymerase Chain Reaction (qPCR) ✔ Realized extractions of total RNAs for 3'RNA sequencing- In vivo skills :Performing in vivo testing in order to assess the therapeutic effect, the biodistribution and to assess the potential toxicity of new product candidates of Transgene in immunocompetent mouse models.✔ Mastered the different types of injections in mice (subcutaneous, intravenous, intra-tumoral and intra-peritoneal)✔ Performed per os administration of research products in mice✔ Performed weight monitoring and daily clinical examination in mice✔ Realized of 3D measurment of tumor volume in mice✔ Performed exsanguination and harvest of all organs and tumors in mice✔ Used GraphPad Prism in order to make different types of graphics (survival, tumor evolution ...)

Région De Montpellier, France

My work consists in assessing gaz tolerance in mice and Caenorhabditis elegans in order to replace an actual greenhouse gaz (SF6). ✔ planned and performed acute inhalation toxicity testing on mice✔ planned and performed carcinogenicity and mutagenicity testing on mice and C. elegans ✔ did a daily follow up of the variation for body weight change and daily clinical exam of mice✔ administered intraperitoneally test substances in mice✔ performed cardiac puncture blood sampling on mice✔ performed tissues and organs collection (brain/liver/kidneys/lungs/adipose and muscle tissue) on mice✔ manipulated, synchronized, counted and observed different phenotype of C. elegans✔ prepared cell culture and microbiological culture✔ prepared protocols, operational procedures, study reports, graphs, and analysis of research data✔ communicated results at team meetings✔ contributed to project and experiment planning✔ contributed in training two internship students

Montpellier Area, France

My mission consists in developing a technique for preparing Zebrafish larval samples for a bioanalysis test as part of a test assessing the risk of convulsant potential of a product in Zebrafish larvae.✔ developed and maintained protocols and operational processes✔ prepared and administered research chemical products on Zebrafish larvae ✔ performed experiment testing on Zebrafish larvae in order to assess the convulsant effect of the company’s research products (using a « video tracking » system)✔ contributed on the improvement of the bioanalysis testing technique , in order to detect the presence of a product in Zebrafish larvae✔ prepared E3 medium for Zebrafish embryos ✔ achieved the Good Laboratory Practices (GLP) formation✔ prepared study reports, graphs, and analysis of research data✔ communicated regularly results at team meetings✔ contributed to project and experiment planning

Région De Montpellier, France

My mission consists in determining the anti-hypertensive activity of Camargue colored rice in spontaneously hypertensive rats (SHR).✔ performed animal husbandry and handling of spontaneous hypertensive rats (SHR) and Wistar rats✔ measured rat's blood pressure using a non invasive blood pressure system✔ injected compounds using intraperitoneal and oral dosing on rats✔ collected, prepared and tested samples using Enzyme-linked Immunosorbent Assay (ELISA)✔ conducted and supported scientific investigations✔ performed statistical analyzes of research data using student t-test✔ communicated results at team meetings

Master 2 (M2), Non Clinical R&D Of Healthcare Products (Pharmacology/Toxicology), With Honors

Level 1 - Animal Experimentation Accreditation (Design And Realization Of Experimental Procedure), Pharmacology And Toxicology

Information Technology

Autodidacte, j'ai de bonnes connaissances en HTML5 / CSS3 / JavaScript.