Florent Collas Email & Phone Number

Cologne, North Rhine-Westphalia, Germany

Évry, Ile-De-France, France

Scientific accomplishments - Metabolic engineering of acetogenic microorganism for the production of third generation isobutene (from synthesis gas, industrial Waste gas) - Development of a genetic and genome-editing tool box - Development and optimization of cultivation protocols for acetogenes, - Flask to small bioreactor gaseous fermentations - Characterization of the fermentation performances of the new generated strainsOther accomplishments - Supervision and training of Engineers and Master students - Presentation of results to non-scientific audiences - Management of the Master Cell Bank and monitoring of the consumable stocks

Geleen, The Netherlands

Scientific accomplishments - Metabolic engineering of acetogenic and hydrogenogenic microorganisms for the production of third generation isobutene (from synthesis gas or industrial Waste gas) - Development of a genetic tool box - Development and optimization of cultivation protocols for acetogenes, - Gaseous fermentations at the Flask scale - Characterization of the fermentation performances of the new generated strainsOther accomplishments - Supervision and training of Engineers, thechnicians and Master students - Introduction of the GLP - Management of the Master Cell Bank and monitoring of the consumable stocks

Wageningen

Implementation of the CRISPR-cas9 system to the genus Clostridia:- development of genetic tools- generation and characterisation of genome edited strains and characterisation of their phenotypes in batch fermentations.

Wageningen, The Netherlands

Within the Isobutanol Platform of Rotterdam I have studyied the production of isobutanol from spruce and poplar wood pulp hydrolysates prepared from organosolv-derived fractions.My tasks included - the optimization of the enzymatic hydrolysis of lignocellulosic fractions obtained by organosolv- the optimization of the fermentation of hydrolysate sugars into isobutanol using a proprietarytechnology from Gevo inc.More information: http://www.be-basic.org/research/iso-butanol-platform-rotterdam-ibpr.html

Rueil Malmaison

I have been hired by IFPEN to introduce the clostridial genetic activity in their laboratory.At this position, I was supervising two technicians with whom we developed genetic tools dedicated to microorganisms from the Clostridium genus.

Over the past decades, the IBE fermentation (isopropanol, butanol and ethanol) has received a renew interest for the production of fuels or biochemicals from renewable materials. In the present study, we have investigated the IBE fermentation using the natural producer C. beijerinckii NRRL B593 and genetically-modified strains of Clostridium acetobutylicum ATCC 824. In batch culture, C. beijerinckii NRRL B593 was found to excrete 13.2 g/L IBE of which 4.5 g/L was isopropanol. To increase IBE production, the gene coding the secondary alcohol dehydrogenase (s-Adh) of C beijerinckii NRRL B593 and different combinations of genes coding for enzymes active in acetoacetyl-CoA to acetone conversion i.e. acetoacetate decarboxylase (adc) and acetoacetyl-CoA: acetate/butyrate: CoA transferase subunits A and B (ctfA and ctfB) were expressed in the ABE (acetone, butanol ethanol) producer C. acetobutylicum ATCC 824. Results showed that the overexpression of the ctfA and ctfB genes significantly increased both speed and extent of the IBE production while the overexpression of the adc gene had only a little effect. In batch culture, the best mutant (expressing adh, ctfA, ctfB and adc) produced 24.4 g/L IBE (of which 8.8 g/L was isopropanol) and displayed an IBE productivity of 0.7 g/L h. Cultivated in continuous mode at the dilution rate of 0.1 h-1, IBE productivity was increased to 1.7 g/L h IBE. As the IBE mix has been considered as a valuable fuel additive, the transformants obtained are a real step forward towards the development of an industrial IBE process for biofuel production.

Microbiolgy and Molecular Biology (fungi and bacteria).

Doctorate (Bac+8), Microbiology, Molecular Biology And Fermentation Processes, Doctor

Bac+5, Life And Food Science

Bac +2, Classe Preparatoire Aux Grandes Écoles (Cpge) Technologie Et Biologie (Tb)

Doctor Of Philosophy (Ph.D.), Life Science

Activities and Societies: Microbiology, Molecular Biology and Metabolic Engineering