Ali N. Email & Phone Number

Cologne, North Rhine-Westphalia, Germany

• Conducted cloning, PCR, and Gibson assembly techniques to engineer microbial strains.
• Transformed plasmids with multiple foreign genes into host organisms.

Straubing, Bavaria, Germany

• Topic: From Genes to Metabolites: A Comprehensive Examination of CYP71B Gene Clusters in Arabidopsis thaliana.
• Identified three enzyme activities within the Arabidopsis thaliana CYP71B gene cluster and elucidated their roles in camalexin metabolism.
• Investigated stress response in Arabidopsis thaliana using biotic (Pseudomonas) and biotic (mannitol, UV) treatments, and analyzing the resultingmetabolite profiles revealed the regulation of various compounds.
• Metabolic profiling and cluster-spanning PCR analysis of four CYP71B gene cluster deletion mutants demonstrated no functional redundancy.
• Using a variety of molecular biology, including PCR methods, cloning, Agrobacterium, yeast and plant transformation, enzyme assays, HPLC, andMS / MS for comprehensive genetic analysis.

Garching, Bavaria, Germany

• Conducted RNA extraction from plants for PCR and utilized Gibson assembly for plasmid construction.
• Troubleshot PCR challenges, successfully amplifying 3 genes.
• Executed protein expression in E. Coli DH5α.

Campus Straubing

• Enhanced the FNR efficiency with H2, addressing electron transfer limitations in biochemical processes.
• Demonstrated NADP+ reduction by oxidizing ferredoxin, influencing future bioelectrochemical research.

Karlsruhe, Baden-Württemberg, Germany

• Cultivated genetically modified E. coli and Vibrio fischeri strains for toxicity and adhesion tests.
• Optimized growth conditions by developing efficient media preparation protocols to ensure consistent development of Vibrio fischeri.
• Responsibilities included maintaining cryostocks of genetically modified E. coli, assisting in writing microbiology lab protocols, researching literature for optimizing Vibrio fischeri growth with various media, and.

Straubing, Bavaria, Germany

• Conducted aerobic growth experiments comparing wild-type and genetically modified strains.
• Measured anaerobic succinate production in both strains.
• Conducted enzymatic assays to measure glucose, succinate, and acetate levels.
• Analyzed and compared growth and succinate production results.

Straubing, Bavaria, Germany

• Developed a Python-based statistical model to predict chemical compound toxicity for nuclear receptor AhR.
• Conducted rigorous validation using a separate set of compounds to ensure model accuracy.
• Optimized and fine-tuned the model by adjusting parameters and algorithms for enhanced predictive capabilities.

Sialkot, Punjab, Pakistan

• Conducted a range of standard medical laboratory tests, including blood counts, transfusion blood tests, hematology, cytology, and histology.
• Set up, maintained, and optimized workstation systems such as PCs, cash registers, home delivery systems, and their accessories.
• Troubleshot small errors that occurred in programs and PCs.
• Managed tasks, documented daily sales, and finances.

Sialkot, Punjab, Pakistan

• Assisted in regulatory tasks to ensure compliance with relevant regulations and guidelines.
• Reviewed and managed internal data for formal compliance.
• Supported dossier preparation for legal compliance in the regulatory affairs process.

Sialkot

• Organized and managed social events, ensuring smooth execution and engaging experiences for attendees.
• Collaborated with event planning teams to coordinate logistics, secure venues, and manage budgets.
• Developed event concepts tailored to specific target audiences and objectives.
• Coordinated with vendors, sponsors, and volunteers for seamless event operations.

Sialkot

• Conducted examinations in government schools to evaluate education quality.
• Implemented an assessment process to gauge teaching effectiveness and student outcomes.
• Collaborated with school officials to ensure smooth execution of exams.
• Analyzed results to identify areas for improvement within the education system.

Sialkot

• Taught biology and chemistry to high school students, emphasizing comprehensive understanding and mastery of key concepts.
• Developed engaging lesson plans with interactive teaching methods and hands-on experiments to enhance student learning.
• Designed curriculum-aligned materials to support students' preparation for Board Exams in biology and chemistry.

Master'S Degree, Chemical Biotechnology

Applied Microbiology and Metabolic Engineering, Artificial Intelligence for Biotechnology, Enzymatic Biotransformation, Protein Chemistry.

Bachelor'S Degree, Biotechnology, B+

Activities and Societies: I have been active in organizing many sports events, blood donation campaigns, technical events etc.Cell.