Frank Benjamin Email & Phone Number

-Process development and scale up of upstream and downstream processes to pilot scale using 500L batch reactors, AKTA Pilot, and continuous flow sonication-Perform QC of purified proteins, protein determination, SDS/Blue-Native gel electrophoresis

• Preform and document following Batch Records and SOP's.
• Operate in pharmaceutical cleanroom environment for production of assemblies, buffers, and downstream product.
• Execute cation exchange, anion exchange, and hydrophobic interaction chromatography
• Perform weighing and dispensing of raw materials for buffer preparation.
• Operation of pH, conductivity, tube welder, pumps and filter integrity testers.
• Experience with chromatography equipment such as AKTA Ready, Pilot, TFF, HIC column, CEX column and AEX.

• Operate 36L Bellco Bioreactors
• Transient transfection and reagent production using G22 and CAT-S cell lines
• Plasmid transformation and purification

• Operated 200L single-use bioreactors
• Operated bench top equipment including pH, conductivity, pumps, tubing welders, and filter integrity tests
• Produced AAV at the 200L scale
• Performed the preparation for buffer and media solutions
• Performed daily cell analysis including, cell counts, viability, cell diameter, and pH
• Document and record cGMP data for processing steps and following standard operating procedures

• Manufacturing and Development Department (40 hours per week during summer/ winter, part-time during semester) June 2019-Present
• Performed day-to-day Manufacturing and Development (upstream) operations such as setting up, sampling, feeding, harvesting bioreactors, centrifugation, depth filtration, making bioreactor assemblies, processing.
• Performed downstream operations (Free Thiol, A280, residual HCP, residual ProA, Bio-A, making HPLC buffers)
• Supported viral clearance studies (packing and testing omni-flex columns with various chromatography resins)
• RAM-managed equipment allocation software that coalesced all certifications into an online program that allowed a user to see all current pieces of equipment and their respective states of calibrationQuality Control.
• Worked on/developed the free thiol assay

• Undergraduate project: Developing a Quality Control Method: Caffeine in Tea
• Used GC-FID to analyze components of green tea, trained on GC-FID, GC-MS IR-spec, HPLC, ESI MS

Gaithersburg, Maryland, United States

• Primarily ran capillary electrophoresis in order to understand antibody fragmentation
• Received in-depth training on two platforms, AbSciex Pa800 plus/ Protein Simple Maurice
• Trained on Empower data processing program
• Primary Project was to compare the new platform;Protein Simple Maurice to the more established platform; AbSciex Pa 800 plus
• Selected Publication: Co-Author: A Tale of Two CE-SDS Platforms: A Head to Head CE-SDS Assessment of the Ab Sciex PA800 Plus and the ProteinSimple Maurice Using Multiple Biologic Compounds

Master Of Business Administration - Mba

Chemistry