Frank Benjamin Email & Phone Number

-Process development and scale up of upstream and downstream processes to pilot scale using 500L batch reactors, AKTA Pilot, and continuous flow sonication-Perform QC of purified proteins, protein determination, SDS/Blue-Native gel electrophoresis

Preform and document following Batch Records and SOP's.• Operate in pharmaceutical cleanroom environment for production of assemblies, buffers, and downstream product.• Execute cation exchange, anion exchange, and hydrophobic interaction chromatography • Perform weighing and dispensing of raw materials for buffer preparation.• Operation of pH, conductivity, tube welder, pumps and filter integrity testers.• Experience with chromatography equipment such as AKTA Ready, Pilot, TFF, HIC column, CEX column and AEX.• Request logbooks and MPRs from Quality Assurance Document Control.• Submit samples for cleaning and in process to Quality Control.• Training on Pathwise and deviation management.• Work with Materials Control to process pick lists and assure all materials are available

• Operate 36L Bellco Bioreactors • Transient transfection and reagent production using G22 and CAT-S cell lines • Plasmid transformation and purification

• Operated 200L single-use bioreactors • Operated bench top equipment including pH, conductivity, pumps, tubing welders, and filter integrity tests • Produced AAV at the 200L scale • Performed the preparation for buffer and media solutions • Performed daily cell analysis including, cell counts, viability, cell diameter, and pH• Document and record cGMP data for processing steps and following standard operating procedures

Manufacturing and Development Department (40 hours per week during summer/ winter, part-time during semester) June 2019-Present• Performed day-to-day Manufacturing and Development (upstream) operations such as setting up, sampling, feeding, harvesting bioreactors, centrifugation, depth filtration, making bioreactor assemblies, processing bioreactor samples making buffers, running titer samples on HPLC, sampling/splitting flasks in a biosafety cabinet• Performed downstream operations (Free Thiol, A280, residual HCP, residual ProA, Bio-A, making HPLC buffers)• Supported viral clearance studies (packing and testing omni-flex columns with various chromatography resins)• RAM-managed equipment allocation software that coalesced all certifications into an online program that allowed a user to see all current pieces of equipment and their respective states of calibrationQuality Control Department (40 hours per week) June 2018-February 2019 • Worked on/developed the free thiol assay • Performed day to day QC operations (balance calibrations, making buffers, pH calibrations)• Carried out stability testing (Bio-A, making HPLC buffers, Free Thiol, Osmolality and pH testing, cell-based potency assay, endotoxin).

• Undergraduate project: Developing a Quality Control Method: Caffeine in Tea • Used GC-FID to analyze components of green tea, trained on GC-FID, GC-MS IR-spec, HPLC, ESI MS

Gaithersburg, Maryland, United States

• Primarily ran capillary electrophoresis in order to understand antibody fragmentation • Received in-depth training on two platforms, AbSciex Pa800 plus/ Protein Simple Maurice • Trained on Empower data processing program• Primary Project was to compare the new platform;Protein Simple Maurice to the more established platform; AbSciex Pa 800 plus • Selected Publication: Co-Author: A Tale of Two CE-SDS Platforms: A Head to Head CE-SDS Assessment of the Ab Sciex PA800 Plus and the ProteinSimple Maurice Using Multiple Biologic Compounds

Master Of Business Administration - Mba

Chemistry