Dedicated, enthusiastic and independent research technician with more than 30 years of experience within culturing of cells, cell biology, assay development and flowcytometry. Core competences:• Assay development: FACS, purifications of cell-subsets, neurons, immunoassays, MAPP assay, purifications of peptides• Cellular assays: purifications, freezing, culturing, proliferations assay, Elispot, MAPP assay• MACS: AutoMACS and manual separation of subsets• Flow cytometry: surface- and intracellular staining, EdU, sorting, QC, maintenance• Molecular biology: DNA/RNA purification, qPCR• Neurons: culturing, staining with antibodies• Immunoassay: ELISA, MHC II binding assay, CBA• Imaging: neurons and PBMC• Purification of peptides: preparation of STAGE tips and manual purification• Histology: cryo and paraffin embedding, staining, immunohistochemistry• Laboratory animals: removal of organs and blood, proliferations assay, cytokine measurement, FACS• Documentation in English in ELNI am used to handle many different tasks, working with deadlines and I am adaptable to shifting working environments. I enjoy working in teams and I function as a good team player. However, I also work well independently and plan my own working day.I approach my tasks with enthusiasm and dedication. I take ownership and responsibility for my tasks in order to reach, not just my own, but also the team goals.I thrive to get new assignments and implement new techniques and assays. I have technical flair, solving problems and acquire knowledge within complexed scientific areas gives me positive energy.I am a fast learner, curious and have an eye for details. I use this combined with my experience when implementing new techniques and instruments to put me thoroughly into issues in order to get the best possible result.