Protein purification and characterisation for structural biology studies (contract, ending 30th June 2021)

Investigation of ELISA repeatability/variation causal factors and their mitigation (contract position ending June 2018)

Protein purification and characterisation for structural biology studies (temporary part time contract, ending 06-04-2016)

Utilisation of established protein and antibody purification and characterisation skills for both Technical Process Development and investigations within CAPA (Corrective and Preventive Actions) cGMP environment CAPA: Investigated process errors, non conformancies and deviations using root cause analysis toolsRisk Management: Prepared and updated Failure Mode and Effects Analysis (FMEA) documentationValidation: Undertook experimental work and contributed to… Show more Utilisation of established protein and antibody purification and characterisation skills for both Technical Process Development and investigations within CAPA (Corrective and Preventive Actions) cGMP environment CAPA: Investigated process errors, non conformancies and deviations using root cause analysis toolsRisk Management: Prepared and updated Failure Mode and Effects Analysis (FMEA) documentationValidation: Undertook experimental work and contributed to characterisation reports in support of test method validationsPlanned and performed process improvement projects, including column bioburden, TFF integrity and affinity column life extensionAnalysed antibodies and antigens using SDS-PAGE, Western / Dot blotting, A280 / BCA quantificationand HPLC (GPC and affinity)Courses attended: SYS2 - Advanced UNICORN system control for chromatography systems (GE Healthcare) Show less

Led and delivered the purification of monoclonal antibodies and recombinant proteins for internal research unitsKey Achievements:Delivered numerous purified recombinant proteins at the milligram scaleIndependently evaluated and successfully established a novel robotic small scale antibody purification technology to supply >100 purified antibodies to therapeutic areas resulting in a 75% time savingDelivered 18 purified confirmed functional monoclonal antibodies from… Show more Led and delivered the purification of monoclonal antibodies and recombinant proteins for internal research unitsKey Achievements:Delivered numerous purified recombinant proteins at the milligram scaleIndependently evaluated and successfully established a novel robotic small scale antibody purification technology to supply >100 purified antibodies to therapeutic areas resulting in a 75% time savingDelivered 18 purified confirmed functional monoclonal antibodies from hybridoma supernatants at the litre scaleProject managed delivery of products to synchronise with follow on assays with all the Sandwich RU'sHeaded safety delegate and incident investigator roles with successful completion of IOSH Safety Delegate certification procedure Show less

An accomplished, successful and valued Protein Purification Scientist, led and delivered purified and characterised proteins generated from multiple expression systems, including mammalian, insect cell, yeast and bacteria to resource high throughput screening, structural studies, biophysics and antibody generation, spanning all Sandwich research unitsKey Achievements:Developed numerous novel purification protocols and assessed literature purification procedures to generate pure… Show more An accomplished, successful and valued Protein Purification Scientist, led and delivered purified and characterised proteins generated from multiple expression systems, including mammalian, insect cell, yeast and bacteria to resource high throughput screening, structural studies, biophysics and antibody generation, spanning all Sandwich research unitsKey Achievements:Developed numerous novel purification protocols and assessed literature purification procedures to generate pure proteins from both native and recombinant sourcesSucceeded in detergent extraction of a membrane protein, refolding from inclusion bodies and Fab generation from antibodiesGood awareness and some experience of working within molecular biology, awareness of aseptic technique and expression, including some cloningAchieved success in protein crystallisation and co-crystallisation, evaluating techniques such as microseedingAugmented protein purification and biochemical characterisation skillsImproved several published purification yields two-fold by protocol modernisation and streamliningPresented results clearly and confidently in group meetings and also more widely in inter-site teleconferences, e.g. protein purification discussion group Show less