Gregory Patton Email & Phone Number

• Lead a group of scientists that focuses on the development of NEB reagents for amplification applications such as PCR, qPCR/RT-qPCR, cDNA synthesis, and loop-mediated isothermal amplification (LAMP).
• Provide technical assistance for the amplification product line to customers, sales staff, international subsidiaries and international distributors.
• Assist the marketing department with content generation, education collateral and maintenance of literature in the form of app notes, webinars, podcasts, blogs, manuals, product brochures, general marketing materials.

• Leader of the custom product development team, which focuses on optimizing and customizing existing NEB products to meet specific customer needs (e.g., lyophilization compatible reagents). Supported over 30 product.
• Established and maintained multiple business relationships simultaneously through direct engagement with customers. Determined their technical requirements and outlined potential customization options while liaising.
• Facilitated adoption of RT-LAMP as an alternative amplification technology for detection of infectious diseases such as SARS-CoV-2 while simultaneously supporting the Luna RT-qPCR product portfolio. Responsible for.
• Lead developer for the SARS-CoV-2 Colorimetric LAMP Assay Kit (E2019), which eventually would be used for workplace surveillance at NEB: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268692
• Managed and mentored team members, both vertically and horizontally, to achieve project objectives and deadlines while also supporting their ongoing professional growth.

Leader of the custom product development team, which focuses on optimizing and customizing existing NEB products to meet specific customer needs.

Ipswich, MA

• Collaborated with the amplification development team to launch the Luna qPCR (M3003/M3004) and RT-qPCR product line (E3005/E3006/E3007)
• Focused on the development of amplification-based products for isothermal amplification, multiplex PCR, qPCR, RT-qPCR and reverse transcription.

Lab Central - Cambridge, MA

• Expressed libraries of single domain antibodies (VHHs) in Escherichia coli and evaluated their ability to bind predictive biomarkers for tumor immunotherapy using flow cytometry and bio-layer interferometry (BLI)
• Developed and optimized sortase conjugation chemistry to site-specifically label VHHs with radionuclides for their use in PET imaging

• Developed and maintained business opportunities to deliver innovative new products while driving organizational growth
• Spearheaded collaborations with innovative partners
• Prepared, negotiated and executed scientific proposals and agreements
• Assisted in the development of a lyophilized next-generation sequencing kit that detects gene fusion events responsible for lung adenocarcinoma (ALK/RET/ROS1)

• Utilized technical skills in molecular biology and biochemistry to function as a project leader within a cross-functional team to develop six new catalog enzyme products under ISO 13485
• Effectively managed a productive team of three research associates to launch a lyophilized next-generation sequencing whole genome library construction kit under aggressive timelines
• Established assays to evaluate new enzyme and NGS products, drafted SOPs, and trained QC analysts to execute quality control analysis assays
• Presented at a quarterly company meeting to educate all employees on the importance of next-generation sequencing while teaching the scientific operations team about current sequencing technologies
• Cloned genes of interested, evaluated and optimized expression as well as solubility of enzymes for scale up in E. coli
• Analyzed and compiled data to present findings at team project meetings

See Product Development Scientist II description above

• Established the mechanism of guanosine monophosphate reductase (GMPR) using enzyme kinetics, mutagenesis, radiolabeling, NMR, MS, and stopped-flow spectroscopy
• Constructed a guaC gene (GMPR) knockout strain of Escherichia coli to prevent wild-type contamination of mutated GMPR proteins during expression in E. coli
• Synthesized the substrate analogs 2-chloroinosine 5’-monophosphate and NADPD as mechanistic probes of GMPR

Urbana-Champaign, Illinois

• Manipulated gene encoded peptides using molecular biology techniques to screen the substrate specificity of lantibiotic synthetases
• Expressed and purified insoluble peptides using affinity chromatography and RP-HPLC to assay lantibiotic synthetase activity
• Evaluated the chemical mechanism of cyclization by lacticin 481 synthetase using mutagenesis, chemical modification, MALDI-TOF MS, and metal analysis
• Engineered and developed novel antibiotic compounds
• Trained incoming undergraduates, graduate students and postdoctoral fellows on laboratory projects and procedures

Phd, Chemistry

Activities and Societies: American Chemical Society, Frontiers in Organic Chemistry Symposium Committee, Educating Tomorrow's Chemists.

Bs, Chemistry

Activities and Societies: American Chemical Society Student Affiliate, Miami Chemical Society, Miami University Steel Drum.