Han Phan Email & Phone Number

Raleigh, Nc, Us

Raleigh, North Carolina, United States

Investigation of transitional metals-dependent enzymes involved in important biological process, including cell proliferation, tRNAs modification, and para-aminobenzoate (pABA) biosynthesis:• Characterized and studied mechanisms of Chlamydia protein associating with death domains (CADD), a manganese-iron oxygenase involved in the biosynthesis of pABA in Chlamydia trachomatis, and tRNA uridine(34) hydroxylase (trhO) involved in tRNAs modification in E.coli.

Vietnam

Carry out individual and collaboration research projects: "Starch hydrolysis by CuSO4/ascorbic mixture”, “Structure of the copper active site in polysaccharide monooxygenases”, “Sizing and Quality discrimination of whiteleg shrimps basing on hyperspectral imaging”, and “Fructosyl-Valine-Oxidase-Based Sensor for electrochemical diabetes assay”• Optimizing highly sensitive methods for determination of glucose generated in starch hydrolysis• Investigating the synergy between CuSO4/ascorbic acid and glycoside hydrolases• Analyzing X-ray absorption spectroscopy (XAS) and electron paramagnetic resonance spectroscopy (EPR) data to gain insights into the copper active site of polysaccharide monooxygenases (PMOs)• Setting up a hyperspectral imaging system, preparing whiteleg shrimp samples, and performing imaging experiments• Expressing, purifying recombinant proteins of interests in E. coli BL21 (DE3) • Catalytic studying enzymes of interests by analytical instruments (HPAEC-PAD, HPLC, GC-MS) and computational software (GROMACS)

The Coordination Chemistry Laboratory

Synthesis, Structural Investigation and Catalytic examination of Copper coordination compounds involving in pretreatment biomass of hydrolysis process.• Verified the starch hydrolysis activity of CuSO4/ascorbic acid• Optimized the hydrolysis conditions using experimental design and response surface method involving multiple parameters

Tang Group, Aie Research Laboratory

Designing fluorescent probes for imaging biological metals via the principles of Aggregation-induced emission (AIE)• Conducted the multi-step synthesis and purification of designed fluorescent compounds• Characterized the synthesized compounds using 1H-NMR spectroscopy