Hernan Cortez Email & Phone Number

Sacramento, Ca, Us

Sacramento, California Area

• Play integral role in Next-Generation Sequencing (NGS)• Perform DNA and RNA extractions on various plant and animal tissues, human blood and saliva samples, and different types of cell lines• Analyze integrity and purity of RNA and DNA samples with Agilent 2100 Bioanalyzer and agarose gel electrophoresis respectively • Library construction using Beckman Coulter Biomek FXP and i7 Laboratory Automation• Library QC utilizing fragment analyzer to measure library fragment sizes • Data entry along with data analysis, filling out reports, and comply with GLPs and SOPs • Lab employee of the year for 2019

Belmont, Ca

• Collaborated in generation of therapeutic monoclonal antibodies for cancer treatment• Generated hybridoma cells by performing electro cell fusion of lymphocytes/splenocytes with myeloma Sp2/0 cells utilizing Nepa Gene ECFG21 cell fusion generator• Maintenance of HEK 293, CHO, hybridoma, and SP2/0 cells• Validated antibodies through ELISA and flow cytometry with Intellicyt iQue Screener• Checked mouse plasma antibody titer through ELISA or flow cytometry • Performed data entry along with data analysis and complied with GLPs and SOPs

Woodland,Ca

• Executed sterility tests to check for the presence of pathogenic bacteria in seed samples via aseptic technique and incubation using Petri dishes• Streak plating of possible contaminants along with conventional PCR analysis• Greenhouse work such as plantings, plant inoculations, and monitoring plant symptoms• Performed data entry (LIMS) and complied with GLPs and SOPs

Williams, Ca

• Conducted sterility tests to check for bacteria in tomato paste samples via aseptic technique using Petri dishes, broth media, and bioMérieux BacT/ALERT 3D System• Classified suspect contaminant bacteria by streak plating, gram staining, and microscopy • Performed data entry, prepared media, and measured chlorine levels of flume water• Conducted BRC standard sanitation tests by collecting swab samples from manufacturing equipment and plating the samples on petrifilms• Complied with cGMPs, GLPs, HACCP, SOPs, and plant safety policies

Davis, Ca

• Participated in generation of reagent monoclonal antibodies for neuronal targets• Ran SDS-PAGE gels, performed western blots, and operated Agilent BioCel for ELISA• Conducted cell culture including subcloning and maintenance of hybridoma cells, freezing and thawing hybridomas, and transfecting COS cells with cDNA of target• Data entry and data analysis, cleaned/autoclaved lab equipment, and prepared reagents

Colusa, Ca

• Collaborated with filing documents, taking phone calls, and preparing documents in the Staff Development Department• Aided in organizing senior health fair by gathering and informing participants • Assisted in organizing CPR classes and organizing employee orientations• Received opportunities to observe anesthesia and surgical procedures in the OR

Williams, Ca

• Deli- provided customer service while being cashier and also preparing sandwiches• Stock boy- helped customers and stocked various items within the deli area

Bachelor’S Degree, Pharmaceutical Chemistry

• Dean’s List-Winter and Spring quarters of 2012 • Neuroscience minor

High School

• Graduated 2nd in class of 75 students (Salutatorian)