Holly Mcphail Email & Phone Number

Richmond, Va, Us

Richmond, Virginia, United States

Richmond, Virginia, United States

• Played integral and resourceful role in supporting team members with all ongoing projects. Inevitable project goal shifts created opportunities to enhance my versatility and flexibility• LC/MS: Sample prep, load and run, data analysis, familiarity with troubleshooting• Collaborated with a co-worker to establish a protocol for Agrobacterium-mediated transformation in 9 varieties of duckweed. Designed and implemented experiments to identify the most proficient media for long term tissue… Show more • Played integral and resourceful role in supporting team members with all ongoing projects. Inevitable project goal shifts created opportunities to enhance my versatility and flexibility• LC/MS: Sample prep, load and run, data analysis, familiarity with troubleshooting• Collaborated with a co-worker to establish a protocol for Agrobacterium-mediated transformation in 9 varieties of duckweed. Designed and implemented experiments to identify the most proficient media for long term tissue propagation, calli induction, frond regeneration, and production of transgenic tissue in duckweed Show less

Richmond, Virginia Area

• Performed Agrobacterium transformations, PCR, Gel electrophoresis, RNA extractions, cDNA synthesis, qPCR, and DNA Sequencing• Conducted whole-plant, Agrobacterium-mediated tobacco transformation in tissue culture experiments with <1% contamination• Made all media necessary for tissue culture experiments• Optimized the tobacco transformation tissue culture process in multiple varieties and reduced number of days to shoot production from 8 weeks to 4 weeks•… Show more • Performed Agrobacterium transformations, PCR, Gel electrophoresis, RNA extractions, cDNA synthesis, qPCR, and DNA Sequencing• Conducted whole-plant, Agrobacterium-mediated tobacco transformation in tissue culture experiments with <1% contamination• Made all media necessary for tissue culture experiments• Optimized the tobacco transformation tissue culture process in multiple varieties and reduced number of days to shoot production from 8 weeks to 4 weeks• Trained all new employees in the craft of tobacco transformation in tissue culture and help to improve their knowledge and skill in aseptic technique• Lab equipment maintenance and calibrations• Provided results through data collection and analysis Show less

Richmond, Virginia Area

Durham, Nc

• Sent to BASF sites in Belgium and Germany to evaluate soybean production and shipping processes and aid in collaborative efforts for improvements• Coordinated experiments across multiple teams to ensure timely delivery of soybean plant material to meet project milestones and deadlines. Facilitated daily correspondence and communications with global team members• Recorded and analyzed data in a variety of digital formats including MS Office and the Laboratory Information Management… Show more • Sent to BASF sites in Belgium and Germany to evaluate soybean production and shipping processes and aid in collaborative efforts for improvements• Coordinated experiments across multiple teams to ensure timely delivery of soybean plant material to meet project milestones and deadlines. Facilitated daily correspondence and communications with global team members• Recorded and analyzed data in a variety of digital formats including MS Office and the Laboratory Information Management System (LIMS)• Presented project updates and other pertinent information to audiences of 150 people using MS PowerPoint• Selected to train European colleague in the art of soybean transformation. Successful in transferring knowledge and skill globally • Selected to enter and QC data from field notebooks for the company's highest priority GLP study • Ordered and received chemicals in Regulatory Science Lab according to GLP practices• Chosen to organize and prepare colleagues for lab and greenhouse Stewardship audit Show less

Durham, Nc

• Performed whole-plant, Agrobacterium-mediated soybean transformation in tissue culture experiments. Propagated soybean plantlets; shipped rooted plantlets to Germany for inoculation and screening; Communicated and coordinated regularly with team members in Germany for allocation of resources• Authored SOPs for soybean plant propagation and shipping internationally according to USDA (APHIS) and BASF Plant Science Stewardship guidelines. Trained and transferred methodology globally… Show more • Performed whole-plant, Agrobacterium-mediated soybean transformation in tissue culture experiments. Propagated soybean plantlets; shipped rooted plantlets to Germany for inoculation and screening; Communicated and coordinated regularly with team members in Germany for allocation of resources• Authored SOPs for soybean plant propagation and shipping internationally according to USDA (APHIS) and BASF Plant Science Stewardship guidelines. Trained and transferred methodology globally • Streamlined transgenic soybean plant propagation and shipping of transgenic plant material internationally and by doing so, reduced the amount of resources previously necessary by 50% • Improved soybean transformation protocol, using a new germplasm, from 6% to 12% transformation efficiency within 6 months Show less

Durham, Nc

• Produced high quality plant material for research, which included soybeans, maize, sunflower, sugar beets, canola, sorghum, potatoes, and medicago• Successfully implemented the use of a short-day photoperiod in the growth of transgenic and non-transgenic soybean plants that decreased the number of days from transplant to seed harvest from 240 to 120 days • Production process required day-to-day operations of two greenhouses and five walk-in growth chambers; included… Show more • Produced high quality plant material for research, which included soybeans, maize, sunflower, sugar beets, canola, sorghum, potatoes, and medicago• Successfully implemented the use of a short-day photoperiod in the growth of transgenic and non-transgenic soybean plants that decreased the number of days from transplant to seed harvest from 240 to 120 days • Production process required day-to-day operations of two greenhouses and five walk-in growth chambers; included trouble-shooting, safety protocol enforcement, work order requests/general maintenance, and pesticide applications• Increased the survival rate of newly transplanted transgenic soybean plants in the greenhouse, from 70% to 94%, within 4 months by experimenting with different types of media, fertilizer combinations, watering regimens, and light intensities• Served as Plant Biology Tech Center representative for multiple high priority projects. • Data entered and tracked in LIMS database and MS Excel. Analyzed data and provided projects with clear and concise results Show less

Cary, Nc

• Herbicide seed treatment of cotton, canola, wheat, and rice; Planted treated seeds in various media to observe percent germinated and herbicide movement• Performed enzyme assays on cotton and canola• Volunteered to help in field spray trials of turf and cotton mapping in the field when needed

Clinton, Md

• Designed and installed seasonal plantings at all high visibility locations; gated entrances and ceremonial lawns• Originated estimates and proposals for projects outside of governing contract• Facilitated weekly meetings with government inspectors to accommodate requests and address concerns• Mixed and applied pesticides on all paved surfaces, mulched beds, and turf areas on 9 of the largest military installations in the Washington, D.C. Metro area

Merrifield, Va

• Assisted customers in plant selection based on their landscape attributes such as sunlight, drainage, soil composition, etc.• Organized herbaceous and woody plants to aid in ease of customer plant selection • Insect inspection and identification weekly for proper Insect Pest Management