James Wareham Email & Phone Number

Developed a prototype web application and data processing pipeline using React and Flask to track and store user interactions.- Generated unique visitor identifiers with FingerprintJS, secured with cryptographic hashing- Handled high concurrency with precise timestamped data storage (JSON)- Optimized server-side logic for scalability and maintained clean, modular code structure

I contributed to the design and implementation of high-throughput methodologies for the separation and detection of small molecules in complex matrices.My primary focus was on expanding and refining scheduled multiple reaction monitoring (sMRM) methods, at times constructing them from scratch. This involved the optimization of a myriad of LC/MS acquisition parameters, maximizing the resolution/signal of hundreds of transitions in a single injection.I became well versed in building robust quantitation methods, using software to configure integration, internal/external standards, and quantitative/qualitative criteria. Specialized scripts would reference these criteria, augmenting the functionality and efficiency of the data review process. My experience running/troubleshooting these scripts provided me a basic familiarity with XML/VBScript.I played a key role in the advancement of our industry-leading Novel Psychoactive Substances (NPS) assay, by: assisting in the development of a dynamic pooling method, drastically reducing turnaround time while maintaining data integrity; and identifying a more reliable internal standard for designer benzodiazepines, improving accuracy by minimizing matrix interferences.I also processed scheduled and certified reference materials and improved extraction/derivatization procedures for drug-drug interaction/chiral resolution assays.With clients nationwide, the NPS assay also gave valuable insight into the distribution and composition of illicitly-manufactured species, allowing us to contribute to a better understanding of the substance use epidemic (see below). We provided pro bono testing to healthcare providers at mental health and harm reduction clinics in underserved areas -- an initiative which highlighted the pressing need for grants to acquire modern instrumentation, offering a humane alternative to historically punitive testing. The data compiled from our NPS studies have been presented at professional conferences.

Nashville, Tennessee, United States

Specialized as a mass spectrometrist, characterizing oral fluid and blood samples in a laboratory offering clinical toxicology assays to healthcare providers.Operated and maintained a fleet of LC-MS/MS (range of Agilent/Sciex systems/software), GC-FID, and automated ELISA instrumentation. Developed expertise in diagnosing and resolving complex instrument malfunctions, including chromatographic issues, pressure anomalies, and signal problems. Trained and mentored new team members.Utilized liquid handling robots for sample preparation and automated solid-phase extraction. Gained additional experience in manual extraction techniques, including liquid-liquid and solid-liquid extraction. Periodically assisted with preparation of COVID-19 samples for RT-PCR testing.Assessed data in real time, monitoring instrument performance and conducting primary data analysis for assays identifying up to 180 drug analytes per injection. Evaluated each sample for quantitative/qualitative criteria using isotopic internal standards, calibrators, and controls. Preserved patient confidentiality and the security of Protected Health Information (PHI) in accordance with HIPAA regulations.Maintained a strong commitment to expanding my knowledge and deepening my understanding of the field, achieving a promotion to the Research and Development department.

I did research with the Department of Physics as part of the 2019 Summer Undergraduate Research Fellowship in the Sciences (SURFS). My work centered around the refinement of an ion-exchange technique for silver nanoparticle fabrication, as well as the spectroscopic characterization of lead ion-implanted silica. I presented posters on these subjects at the Fall 2019 Summer Undergraduate Research Symposium.I also contributed to proteomic research initiatives within the College of Pharmacy, investigating the development of an accessible methodology for the isolation, purification, and preparation of exosomes from colorectal cancer cells. We analyzed two cell lines with differing 3D morphologies, one cetuximab-resistant and the other cetixumab-responsive.

- Guided fellow undergraduate students in real-time cadaver dissection- Partnered with graduate students with special focus in musculoskeletal dissection

Provided kinesiology, anatomy and physiology, and chemistry support to peers, including athletes and students in the Bridges to Belmont access program.

- Led up to 40 building monitors within the College of Music and assisted during performances.- Handled clerical work as well as game-day operations for D-I sports teams with Belmont Athletics.

Gained combined 120+ hours of clinical experience shadowing surgeon specializing in sports medicine in both exam and OR environments. Cases included quadriceps tendon rupture, hip/knee arthroplasty, meniscus repair, trigger finger release, carpal tunnel release, cheilectomy, subacromial decompression, and various fracture repairs.

Gained combined 120+ hours of clinical experience shadowing surgeon specializing in the spine in both exam and OR environments. Cases included anterior lumbar interbody fusion, anterior cervical discectomy and fusion, laminectomy, and sacroiliac joint fusion.

Late Entry Accelerated Program, Materials Science & Engineering

Bachelor Of Science (Bs), Pharmaceutical Studies

Activities and Societies: Chorale, Club Volleyball, Beta Chi BCMB Club, Student Leaders Promoting Education (SLOPE), Student-Led.

High School Diploma

Activities and Societies: All-State/National Choirs, Madrigals, Vocal Jazz, Jazz Band, Marching Band, Symphonic Band, Orchestra, Theater.