Dr. Alex Green’s Laboratory• High-throughput fluorescence-sorting-sequencing pipeline for characterization of riboregulator devices• Developing a deep learning framework for sequence-to-function prediction of engineered nucleic acid sensors• Designing RNA attenuator systems for transcriptional and translational regulation• Integrating isothermal amplification strategies with a recombinase-based lateral flow strip readoutThe Green lab specializes in studying molecular… Show more Dr. Alex Green’s Laboratory• High-throughput fluorescence-sorting-sequencing pipeline for characterization of riboregulator devices• Developing a deep learning framework for sequence-to-function prediction of engineered nucleic acid sensors• Designing RNA attenuator systems for transcriptional and translational regulation• Integrating isothermal amplification strategies with a recombinase-based lateral flow strip readoutThe Green lab specializes in studying molecular interactions between nucleic acids and proteins to direct the assembly of nanometer-scale organic and inorganic components. Through this work, we construct information-processing circuitry inside living cells for uses in biosensing, nanotechnology, diagnostics, and broader biotechnology applications Show less

ENG BE209: Principles of Molecular Cell Biology and BiotechnologyIntroduction to the molecular, physical and computational principles of cell function in the context of cutting-edge applications in bioengineering and medicine.

Implemented electrochemical strategy for low-cost viral detectiono Demonstrated first use of novel gold leaf electrode for biosensing purposes and first functionalization of pure gold leaf material for detection from HPV 16, HPV 18 clinical sampleso Fabricated flexible, low-cost electrode that use $0.16 of gold using equipment-free fabrication protocol for low-resource settings, with an ~$0.50 cost per device, and ~$2.30/testo Utilized CRISPR-based detection of synthetic viral DNA… Show more Implemented electrochemical strategy for low-cost viral detectiono Demonstrated first use of novel gold leaf electrode for biosensing purposes and first functionalization of pure gold leaf material for detection from HPV 16, HPV 18 clinical sampleso Fabricated flexible, low-cost electrode that use $0.16 of gold using equipment-free fabrication protocol for low-resource settings, with an ~$0.50 cost per device, and ~$2.30/testo Utilized CRISPR-based detection of synthetic viral DNA and HPV 16/18 DNA ratiometric sensing Developed a proof-of-concept prototype to demonstrate DNA capture and enrichment from urine samples using chitosan mediated electrostatic captureo Combined a novel thermophilic helicase dependent amplification (tHDA) test for rapid amplification to process samples in 1-5minutes, amplification in 30minutes and time to result in under one houro Adapted paper-microfluidic platform for colorimetric lateral flow strip readout and validated assay using clinical samples and compared to gold standard to achieve 96% sensitivity and 100% specificityCombined loop mediated isothermal amplification and sample preparation method into a device with visual readout for rapid point-of-care diagnosis of P. falciparum using finger-prick bloodo Integrated DNA/RNA capture, lysis, and elution from complex sample matrices (Blood) with nucleic acid stabilization technique on a stable paper matrix for storage at 22 degrees C for up to two weekso Maintained malaria infected red blood cell culture to keep malaria culture viable for use in experiments Show less

• Optimize a quantum dot fluorescent biosensor design for rapid, instrument-free small molecule sensing to achieve nanomolar concentration visual limit of detection• Design a novel imaging system for biosensor to employ ratiometric sensing through an image processing analysis software

ENG EK 131 -- Hands-On EngineeringIntroduce first year engineering students to engineering analysis and design through work in Boston University's Engineering Product Innovation Center.Instruct on basic concepts like CAD (OnShape, Solidworks), machining (hand tools, CNC machine, lath, laser cutter, etc.), introduction to electric circuits, programming micro-controllers, and product design.

ENG EK210- Introduction to Engineering Design· Conferred with students about assignment and topic clarification; development and organization of tasks/topics; familiarity with many fundamental engineering concepts including 3D Printing, Electric Circuits, rapid prototyping; aided with data acquisition and prototype analysis.

Precision Diagnostics Center | DAMP Lab- Developed automation workflows to increase COVID-19 testing throughput for Boston University through integration of sample preparation and extraction methods with automation hardware like the Hamilton STAR- Oversaw the design and implementation of qPCR assay validation/qualification in support of COVID-19 clinical testing in Boston University's CLIA-certified lab- Authored controlled, technical documents such as SOPs and assay work sheets… Show more Precision Diagnostics Center | DAMP Lab- Developed automation workflows to increase COVID-19 testing throughput for Boston University through integration of sample preparation and extraction methods with automation hardware like the Hamilton STAR- Oversaw the design and implementation of qPCR assay validation/qualification in support of COVID-19 clinical testing in Boston University's CLIA-certified lab- Authored controlled, technical documents such as SOPs and assay work sheets, and trained technologists and technicians on manual and automated sample aliquoting, extraction, and qPCR workflows. Show less

Woodwind Instructor for students aged 8-20 on Saxophone, Clarinet; teach concepts in music theory, technique, musicianship, and ensemble/solo performance

· Collaborated on prototyping strategy, component/design selection, and product launch for medical devices· Implemented design process on product development for medical devices, instrumentation, and equipment, specifically for: a catheter/needle actuation system, infusion set manufacturing/quality control, and a lateral flow diagnostic reader