Jarrod Clark Email & Phone Number

San Diego, Ca, Us

San Diego, Ca, Us

Waltham, Ma, Us

Waltham, Ma, Us

- Used automation equipment (Genetix Q-Pix Robotic Colony Picker and a Beckman Biomek FX liquid handler with a Thermo Scientific Cytomat) as well as LIMS software in a High-Throughput Environment. - Used a library of Gateway Entry Clones to construct, isolate, positively identify, and prepare for shipment, ~6000 Gateway Lentivirus Destination Clones. - Project completed on time and within budget.

Duarte, Ca, Us

Research Projects: - Identified cancer biomarkers for biopsy and Expressed Prostatic Secretion (EPS) specimens - Designed experiments for cancer biomarker detection with real-time QPCR- Designed methylation sensitive sodium bisulfite based assays using real-time QPCR- Constructed plasmid standards (14 biomarkers) for use with real-time QPCR - Ran QPCR on over 1000 clinical samples for four methylation and five cDNA cancer biomarkers- Quantified methylation sensitive restriction digests for genomic methylation state using QPCR- Used bisulfite sequencing and LMPCR to identify DNA breakpoints as potential cancer markers - Isolated methylated sequences from prostate cancer specimens using the methyl CpG binding protein 2 (MECP2) - Using the GE AKTA, purified M.EcoRII and M.HhaI from IPTG based E.Coli expression systems, as well as Human DNA (Cytosine-5) Methyltransferase from HeLa Cells- Optimized a commercially available Progensa assay to detect the PCA3/DD3 cancer biomarker- Through bisulfite sensitivity and TOPO cloning, mapped sites of DNA secondary structure - Performed both methylation sequencing and QPCR quantification of genomic DNA isolated from PC3 cells treated in vitro with 5-AzaC and 2´-Deoxyriboguanylurea- Implemented a protocol to quantify polyamine levels in clinical specimens as a possible cancer biomarker - Performed both molecular modeling and electronic structure calculations- Designed an aminophenyltrimethoxysilane based protocol for binding DNA to glass chips for microfluidicsManagement and Communication:- Trained/supervised colleagues and students (high school to graduate student level)- Assisted in manuscript preparations (including figures illustrations), internal review board protocol development and grant proposal preparation.- Selected and invited for poster and oral presentations- Ordered laboratory supplies and maintained equipment- Laboratory safety manager (chemical, biohazard and radioisotopes)

Duarte, Ca, Us

Research Projects: - Quantified hTR expression to detect prostate cancer using microfluidics chips- Inspected telomerase activity in prostate cancer with the TRAP and TRAPeze assays- Purified human and bacterial DNA methyltransferases- Collected human placentas to purify human DNA methyltransferase 1 (DNMT1)- Performed kinetic studies on methyltransferase inhibitors and substrates- Ran retardation PAGE on 32P labeled oligo substrates and inhibitors of DNA methyltransferases- Designed and created DNA based nano-devices- Perfected a microfluidics based retardation technique for DNA nano-devices - Screened conditions to crystallize the EcoRII methyltransferase- Showed that hDNMT1 does not convert dU to dT and that the donated methyl groups are confined to 5'-methylcytosine

- Performed quantitative hepatitis C assays- Ran Quantitative and qualitative HIV assays using PCR and ELISA - Utilized time management skills in order to perform up to 50 assays/day