Jason Holt Email & Phone Number

Raleigh, NC, US

Research Triangle Park, NC

Point of accountability for the development and delivery of a comprehensive preclinical safety strategy for programs within their designated therapeutic areaMatrix leadership to ensure Translational Medicine resources are effectively deployed to support programs within their designated therapeutic area, management/resolution of safety issuesEnsure delivery.

Durham, North Carolina, United States

Responsible for development of translational biomarkers for both efficacy and toxicity from in vivo models, as well as provide oversight and expertise in evaluation of preclinical in vivo models relevant to specific gene therapy targets.Nonclinical representative in the successful completion of multiple IND filings. Work with external CROs to progress cell.

Durham, North Carolina

Responsible for development of translational biomarkers for both efficacy and toxicity from in vivo models, as well as provide oversight and expertise in evaluation of preclinical in vivo models relevant to specific gene therapy targets.Nonclinical representative in the successful completion of multiple IND filings. Work with external CROs to progress cell.

Raleigh-Durham, North Carolina Area

Director responsible for progressing drug candidates into the clinic, by leading project teams responsible for design and implementation of pre-clinical in vivo pharmacology studies, pharmacokinetic studies, safety and toxicity studies, and Investigational New Drug (IND) enabling activities, while maintaining the budget for all program activities and.

Durham, NC

Project leader on pre-clinical drug discovery program focused on oncology and neuropathy indications.

Durham, NC

Biologist working on lead optimization drug discovery programs for cancer, kidney disease, and other indications.

Durham, North Carolina

Project leader for clinical trial supply chain programs to ensure proper regulatory labeling, distribution, and maintenance of clinical supplies at clinical trial sites. Customers include large international pharmaceutical companies, biotechnology companies, and academic institutions.

Research Triangle Park, NC

• Leader of a matrix team of biologists, chemists, and clinicians in a lead optimization project to investigate the effect of a small molecule inhibitor on the regeneration of skeletal muscle and skeletal muscle.
• Leader of a team of cell biologists, in vivo pharmacologists, chemists, and biochemists to understand the effects of a secreted protein on skeletal muscle regeneration.

Research Triangle Park, NC

• Co-Project leader of Myokine discovery effort in collaboration with Biopharm tBDU in Biopharm R&D.
• Manager of a direct report to support efforts with the LRH-1 nuclear receptor project, myostatin pathways assay, and Santa Fe pathways effort.
• Manager of co-op students and summer intern.
• Co-project leader for HTB Lean Sigma efforts.
• Member of the Harvard Stem Cell Institute team to investigate therapeutic opportunities of skeletal muscle stem cells and skeletal muscle regeneration and repair.
• Developed and ran a cell based screen of new chemical entities in a skeletal muscle stem cell population to be utilized in treatment of skeletal muscle damage and regeneration.

Research Triangle Park, NC

• Developed cell based models for target validation of the Mineralocorticoid receptor for the treatment of cardiac fibrosis.
• Using in vitro cell based assays determined the mechanism of action by which prostaglandin compounds activate NGFIB Beta transcriptional activity.
• Determined the mechanism of action of the growth factor FGF19 in bile acid regulation via differential gene expression and protein biochemistry.
• Developed cell based assays in both muscle and liver cells to measure activity of RORa compounds and used siRNA to validate effects of compounds.

Research Triangle Park, NC

• Worked with team members to clone all full length human nuclear receptors and subclone into mammalian expression vectors.
• Developed Mammalian-Two-Hybrid cell based assay for the Dynamic Stabilization of nuclear receptors, which can be used to screen against compounds to find novel drugs for nuclear receptors.
• Generation of reagents for antibody production to Human PNR.
• Developed full length NGFIB Beta (NOT1) transient transfection assay.
• Developed Gal4 chimera NGFIB Beta (NOT1) transient transfection.
• Developed cell based assays to measure activity of RORa compounds.

• Responsibilities:
• Upkeep of lab DNA construct book.
• Maintenance of radioactivity records.
• Maintain and order stock supplies for the lab.
• Supervise both work study students and summer interns.
• Mating, genotyping, and general maintenance of several knockout mouse colonies. Projects:

Ms, Physiology

Education record