Javier Fernández Fernández Email & Phone Number

Madrid, Community Of Madrid, Spain

Backend web development using Node.js in a NestJS framework and an AWS DevOps cloud environment. API development and integration with SQL and NoSQL databases.

Greater Madrid Metropolitan Area

Development and participation in several integration projects. Maintaining databases and integration services (SAP SCI and SAP HANA).

Éibar, Basque Country, Spain

Soil and water sample retrieving for pollutants analysis, report writing (physical environment description, use of GIS) and project writing (such as maintenance of green areas and advising municipalities).

Bilbao Area, Spain

I carried out my master's final thesis in the EKOFISKO research group, located in the Department of Ecology and Plant Biology in the Faculty of Science and Technology of the University of the Basque Country (UPV/EHU).Here is a short abstract of it:Although not in the Mediterranean Basin, Mt. Teide (3,718 m) in Tenerife, Canary Islands, has a Mediterranean climate even above the treeline and very high visible and UV radiation related to the elevation and latitude (28°N). The… Show more I carried out my master's final thesis in the EKOFISKO research group, located in the Department of Ecology and Plant Biology in the Faculty of Science and Technology of the University of the Basque Country (UPV/EHU).Here is a short abstract of it:Although not in the Mediterranean Basin, Mt. Teide (3,718 m) in Tenerife, Canary Islands, has a Mediterranean climate even above the treeline and very high visible and UV radiation related to the elevation and latitude (28°N). The harsh conditions of the Mediterranean climate and the alpine environment have a combined effect on the flora that develops there, exposing it to several abiotic stresses at the same time. 4 evergreen species, native to the alpine volcanic semi-desert of Mt. Teide, with pubescent (Echium wildpretii and Sideritis eriocephala) and glabrous (Bencomia exstipulata and Pterocephalus lasiospermus) leaves, were selected to study their photochemical dynamics at the end of the winter. Changes in photochemical efficiency, photosynthetic pigment composition, and content of UV-protectant flavonoids were studied to assess their daily dynamics. The results revealed that each species had a particular strategy to confront the harsh conditions of their environment. B. exstipulata presented a high zeaxanthin content at predawn (a time in which plants generally show the minimum value of the day). P. lasiospermus and S. eriocephala presented low content of xanthophyll cycle pool (VAZ) and total chlorophyll content, but their AZ/VAZ was highly variable during the day. No protection from trichomes could be attributed to S. eriocephala leaves, except a lower Chl a/b ratio when compared with other species. Lastly, E. wildpretii leaf trichomes diminished availability of light for the photosynthetic apparatus, which related a low VAZ content and a less responsive AZ/VAZ ratio. Anthocyanin and flavonol contents were similar in the four species, suggesting a more seasonal rather than daily variation pattern. Show less

Elx / Elche, Alicante, Spain

I carried out my bachelor's degree thesis in coatings for silica nanoparticles to protect protein drugs from stomach digestive enzymes and, this way, use them as a vehicle to administrate them orally. I was in the Biothermodynamics of Molecular Recognition Processes Group, Molecular Recognizement and Protein Engineering Subunit (Molecular and Cellular Design Unit).Here is the abstract of it:In recent years, biomedical research has addressed new approaches to the administration… Show more I carried out my bachelor's degree thesis in coatings for silica nanoparticles to protect protein drugs from stomach digestive enzymes and, this way, use them as a vehicle to administrate them orally. I was in the Biothermodynamics of Molecular Recognition Processes Group, Molecular Recognizement and Protein Engineering Subunit (Molecular and Cellular Design Unit).Here is the abstract of it:In recent years, biomedical research has addressed new approaches to the administration of drugs in order to transport drugs to be administered in a simple, painless and cheap way. Oral administration is a challenge when dealing with protein drugs, as these are usually sensitive to intestinal transit (especially in the stomach), being degraded by the different pH of the organs that compose it or by the action of digestive enzymes contained in them. Previously, it has been demonstrated in the laboratory that silica nanoparticles functionalized with 3- (trihydroxysilyl)-1-propane sulfononate (THSPS, SiO2@SO3-) are effective in adsorbing proteins at pH = 1.5 (stomach) and releasing them at pH = 7 (intestine) while keeping theis biological integrity intact.The main objective of this project is to develop methodologies capable of protecting the proteins adsorbed onto SiO2@SO3- nanoparticles from the proteolytic action of the various enzymes that exist along the transit. For this end, we propose the use of different neutral polymer capable of coating the protein and protecting it from proteolytic attack. Using Reduced and Carboxymethylated Lysozyme (RCM-LZM) adsorbed on SiO2@SO3- as a model, we have evaluated the effect of four different neutral polymers (PVP, PVA, Ficoll and PEG 4000) in their ability to inhibit the proteolytic attack by porcine trypsin. It was concluded that both PVP and PVA are able to coat the protein-nanoparticle complex and effectively protect the adsorbed protein from its proteolytic attack. Show less

Valencia, Spain

I participated in the whole protocol of genetic transformation in citrus, in which I carried out the following steps:▪ in vitro culture▪ Agrobacterium tumefaciens transfection▪ in vivo and in vitro grafts in citrusFurthermore, I learnt and practiced various technics of molecular biology:▪ Electrophoresis and PCR▪ gDNA extraction of vegetal samples▪ DNA plasmid extraction from Escherichia coli cultures▪ Use of Nanodrop and basic equipment of… Show more I participated in the whole protocol of genetic transformation in citrus, in which I carried out the following steps:▪ in vitro culture▪ Agrobacterium tumefaciens transfection▪ in vivo and in vitro grafts in citrusFurthermore, I learnt and practiced various technics of molecular biology:▪ Electrophoresis and PCR▪ gDNA extraction of vegetal samples▪ DNA plasmid extraction from Escherichia coli cultures▪ Use of Nanodrop and basic equipment of laboratoryAnd lastly, I carried out more specific experiments that taught me more complex technics:▪ Carotenoids extraction from citrus fruit tissues▪ Preparation of samples to flow cytometry▪ Extraction of volatile citrus compounds Show less

Madrid, Spain

I took part in the start up of an experiment in the Molecular Physiology of Behavior group (Molecular, Cellular and Developmental Neurobiology department) to examine the memory capacity of Drosophila melanogaster mutants for a circadian cycle gene.I also learnt and practices some specific techniques such as:▪ Learning how to use the confocal and fluorescence microscope.▪ Learning the ELISA protocol to visualize imaginal discs in the fluorescence microscope.Lastly, I… Show more I took part in the start up of an experiment in the Molecular Physiology of Behavior group (Molecular, Cellular and Developmental Neurobiology department) to examine the memory capacity of Drosophila melanogaster mutants for a circadian cycle gene.I also learnt and practices some specific techniques such as:▪ Learning how to use the confocal and fluorescence microscope.▪ Learning the ELISA protocol to visualize imaginal discs in the fluorescence microscope.Lastly, I learnt some techniques and knowledges about Drosophila melanogaster:▪ Learning about the care and life cycle of Drosophila melanogaster.▪ Study and dissection of fly larvae to know their different parts, in particular their imaginal discs and brain. Show less

Coding, Informatics

At 42 Madrid Fundación Telefónica, I am learning how to code from the very beginning. Right now, I am learning C and UNIX.

Master'S Degree In Environmental Agrobiology, Plant Sciences, 9.23

The Master's degree in Environmental Agrobiology is a highly research-focused master, but also gives an overall vision of the industrial.

Bachelor'S Degree, Biotechnology

Activities and Societies: During my practical lessons I was part of the ARA Group (High Academic Performance Group) whose main objective.