Experienced Protein Biochemist and Molecular Biologist with work experience and knowledge in recombinant protein purification and characterization. ✭ Effectively managed projects in protein expression and purification (from bacteria and mammalian systems), carried out protein characterization and performed troubleshooting. Validated protein-protein and protein-nucleic acid interactions. Authored 6 lead author publications. ✭ Contributed as an individual and as part of a group to the development of several stable cell lines expressing proteins of interest. Designed and developed constructs for generation of mouse models. The outcome of a knockout mouse model was a highly cited publication (more than 300 citations). ✭ Analyzed, summarized and presented data for poster presentations and publications in peer reviewed journals. Participated in writing grants that brought in $ 1,000,000 of laboratory funding. ✭ Efficient in project management as evidenced through successful collaborations that yielded 5 co-author publications. ✭ For the next step in my career, I am excited to integrate my skills in critical thinking and problem solving towards the discovery/development of therapies to better human life. I am open to new opportunities in an industry setting. Contact: jbdmascarenhas-at-gmail.com or 773 540 4724Bioprocessing: Cell banking, thawing and expansion, production, and harvest.Protein expression (Bacteria and mammalian), purification, and analysis: ELISA, Kinase assays, Western blotting. Functional assays: isozyme assays, enzyme assays, protein-protein interactions, cell-based assays.Tissue : Processing tissue samples for frozen sections and immunofluorescence. Molecular Biology : PCR, RT-PCR, Cloning and Recombineering, Southern blots, Gel shifts, Viral vectors and virus particle generation.Bioinformatics : DNA, RNA and protein structure analyzes.WILLING TO RELOCATE AND WILL NOT NEED VISA SPONSORSHIP.