Provide laboratory services for Next-Generation Sequencing

- Manual and automated RNA and DNA isolations of tissue, blood, serum, plasma, FFPE tissues and cells- Proficient with Thermo Scientific KingFisher® machinery and software- Ensured accurate and detailed records were kept for samples received as well as for solutions and instruments used- Reverse Transcription (RT) and Real-Time PCR of cDNA product- Target-specific RT, and subsequent qRT-PCR amplification of miRNA targets on total RNA samples- Sectioning and staining (H&E) of FFPE tissues- Updated the isolation protocols into Excel forms that automatically populate cells, perform calculations, help decrease unnecessary repetition of handwritten work, increase efficiency with more concise directions, and increase quality with visual checks- General laboratory equipment experience: thermal cycler, real-time PCR machine, bioanalyzer, nanodrop, E-Gel® Agarose System, centrifuges, homogenizer

- Researched the manipulation of plant storage tissue and the use of biotechnology in crop investment- Analyzed the rate of digestion and the amount of resistance starch in different rice samples

- Cared for swordtail fish specimens on weekly basis- Analyzed specific single-nucleotide polymorphisms (SNPs) from Xiphophorus malinche and Xiphophorus birchmanni and compared the hybrid population of these two species