Jimmy Mevaere Email & Phone Number

Ville De Paris, Île-De-France, France

Région De Paris, France

Région De Paris, France

3-month intensive BootCamp at Datascientest.com (Formation Data Science Paris Code):- Python, R, PySPARK- Machine learning, PCA, KNN, Kmean, SVM, random forest, computer vision, NLP ...- data visualization- project: Reinforcement Learning

Mnhn, Paris

My project was focused on lasso peptides (LP), a growing class of the ribosomally synthesized and modified peptides family (RIPPs), that possess a unique topology. They exhibit strong resistance to physical and chemical denaturation and diverse biological activities, thus making them interesting candidate for pharmaceutical and biotechnological purpose.- General molecular biology (cloning, PCR, RNA extraction, RT-PCR, qPCR, site-directed mutagenesis, PCR targeting)- Heterologous… Show more My project was focused on lasso peptides (LP), a growing class of the ribosomally synthesized and modified peptides family (RIPPs), that possess a unique topology. They exhibit strong resistance to physical and chemical denaturation and diverse biological activities, thus making them interesting candidate for pharmaceutical and biotechnological purpose.- General molecular biology (cloning, PCR, RNA extraction, RT-PCR, qPCR, site-directed mutagenesis, PCR targeting)- Heterologous expression (streptomyces)- Peptide production, extraction and purification- Mass spectrometry (LC-MS, ESI Qq-TOF Q-Star Pulsar)- Scanning electronic microscopy (SEM, Hitachi SU3500) Show less

Région De Lyon, France

Set up and use of two mass spectrometry-based approaches in quantitative proteomics: TAILS (Terminal Amine Isotopic Labeling of Substrates) and PICS (Proteomic Identification of protease Cleavage Sites)- Cell culture, protein production and purification, iTRAQ labelling, SDS- PAGE, western blot- Strong cation exchange Chromatography (SCX),- N-TAILS, PICS- Mass spectrometry: nanoLC-MS (Eksigent nano HPLC - ESI-QqTOF, QStar Elite, Applied Biosystems)- Data processing:… Show more Set up and use of two mass spectrometry-based approaches in quantitative proteomics: TAILS (Terminal Amine Isotopic Labeling of Substrates) and PICS (Proteomic Identification of protease Cleavage Sites)- Cell culture, protein production and purification, iTRAQ labelling, SDS- PAGE, western blot- Strong cation exchange Chromatography (SCX),- N-TAILS, PICS- Mass spectrometry: nanoLC-MS (Eksigent nano HPLC - ESI-QqTOF, QStar Elite, Applied Biosystems)- Data processing: Mascot, Protein pilot Show less

Marcoule, France

Development and optimization of proteomic approach for proteome and posttranslational modifications analysis- Biochemistry: Cell Lysis, protein extraction, Sample preparation- Strong cation exchange chromatography (SCX), isoelectrofocusing Offgel (IEF),- Phosphopeptides enrichment (IMAC)- Mass spectrometry: MALDI-TOF, microLC-MS (UltiMate 3000, Dionex - LTQ-Orbitrap XL, ThermoFischer- Data processing: Mascot, Mascot Daemon

University Of Queensland, Brisbane, Australie

Mass spectrometry analysis of cone snail Venom at Richard J. Lewis Group- Peptides purification: Reverse phase HPLC, Radioligand binding assay- De novo sequencing by mass spectrometry of venom peptides- Mass spectrometry: MALDI TOF-TOF (4800, Applied Biosystems), LC-ESI QqTOF (QStar Elite, Applied Biosystems)

Montréal, Canada

Doctor Of Philosophy (Ph.D.), Biochemistry And Molecular Biology

Lasso peptides from Actinobacteria

Master Of Science (M.S.) Biology And Biotechnology (Proteomic), Biochemistry

Granted 5 years after the Baccalauréat, Thesis: “Proteomic approach for PTM analysis of radio tolerant microorganism proteins” Thesis.

Licence Biologie Et Biotechnologie, Mention Génomique Et Protéomique, Biotechnologie, Admis

Dut Génie Biologique, Analyses Biologiques Et Biochimiques

Two-year unviersity degree in technical Science Thesis: “Implication of calcium and calpaïns in apoptosis induced by acrolein”