Jody Toperzer Email & Phone Number

Denver, Colorado, United States

Worked on finding a funding source and writing grant for chosen new disease target for the companies novel immunomodullatory cell-directed extracorporeal selective cytopheretic device (SCD). Designed preclinical and clinical trials for current and future grants.

Aurora, Colorado, United States

● Transformed empty lab with only dirty glassware into a fully functional laboratory space. ● Working with immortalized neutrophils using experimental techniques such as flow cytometry, western blotting, and microscopy. ● Stain and screen samples using multicolor flow cytometry.● Optimized various protocols for lab use.● Organized and documented inventory and protocols.● Keep lab stocked with supplies and sterile materials.● Trained rotating graduate students on SDS-page and western blots.

Buhl, Idaho, United States

● Method development and optimization for pathogen testing in Rainbow Trout using PCR, gel electrophoresis, reverse transcriptase PCR, and real-time PCR.● Assisted in vaccination trials and analyzed gene-expression of the immune system in Rainbow Trout.● Utilized experimental planning, data-analysis, and protocol development skills to expand molecular biology research for the company.● Optimized nucleic acid extractions for various types of samples to achieve the most cost effective and high throughput methods.● Prepared high quality samples for sequencing and conducted sequence data alignment, assembly, and similarity analysis between isolates in Geneious. ● Administered diagnostic services for Rainbow Trout production farms which included gross pathology, sample collection, and sample preparation for Virology, Bacteriology, and Molecular Biology analysis.● Performed interpretation of Bacteriology samples for diagnostic cases. ● Oversaw molecular biology records, inventory, reports, and documentation of results for research experiments.

Knoxville, Tennessee Area

● Collaborated with CASIS, NASA, and SpaceX to successfully perform human cell-line experiments on the International Space Station. ● Researched different applications of autobioluminescent technology in various human cell lines.● Maintained and performed research with human cell-lines: HeLa, HEK293, HepG2, HepaRG, HUVEC, HBEpC, PANC1● Prepared various plasmids using techniques such as restriction digest, ligation, transformation, cloning, and plasmid extraction.● Transfected various human cell lines with autobioluminescent lux plasmids using chemical reagents and electroporation. ● Used a Clariostar plate reader to confirm luminescent signal in transfected cell lines.Proficiently manipulated and examined data in Microsoft Office for data analysis and reporting.● Managed supply ordering and reduced consumable costs by researching and comparing supplies to obtain the most cost effective and beneficial supplies for present and future projects. ● Assisted lead scientists with developing protocols, project planning, method development, and laboratory organization.● Conducted weekly lab meetings with other researchers to exchange project progress and keep workflow smooth and organized.

Dr. Bemebenek Worm Genetics Cell Biology/ Microscopy Laboratory (Fall 2015-2016)Dr. Von Arnim Plant Molecular Biology Laboratory (Fall 2016-Summer 2017)Dr. Wang Diagnostic Oncology Laboratory (Fall 2017- Fall 2017)

Master Of Science - Ms, Biomedical Science & Biotechnology

Bachelor'S Degree, Biochemistry & Molecular Biology