John Basile Email & Phone Number

St Paul, Minnesota, United States

• Working on ultrasound instrumentation/technology towards developing a novel, noninvasive neuromodulation technique that is portable, cheap, and able to be easily used by the end user• Helped develop testing protocols for ultrasound device output measurements, device function longevity, and device safety according to IEC standards and to meet FDA regulation requriements• Testing of multi-element array ultrasound technology to meet performance and safety criteria in device output and longevity• Performing tests of device pressure steering capabilities and output pressure waveform in standard water tank setups including both phantom and ex-vivo tissues• Developed a computational model for tissue heating from our ultrasound devices that has been accepted by the FDA to show safe parameter settings and therapy protocols• Developed a device output testing protocol to determine the pressure waveform focal diameter according to IEC standards

The Sensory Optimization and Neural Implant Coding Laboratory (Dr. Hubert Lim)• Working on ultrasound instrumentation/technology towards developing a novel, noninvasive neuromodulation technique• Experience with animal studies performing chronic stimulation• Experience with perfusion surgeries to quickly and effectively fix tissue• Developed and produced a pressurized perfusion device for surgeries• Experience with histology including sectioning, mounting, staining, and imaging brain tissuesThe Biomedical Functional Imaging and Neuroengineering Laboratory (Dr. Bin He)• Experience with recording and analyzing electrophysiological data from small rodents including EEG, LFP, and spiking data • Signal processing included developing denoising programs for the collected raw data • Worked on ultrasound instrumentation/technology towards developing a novel, noninvasive neuromodulation technique• Developed and utilized an ultrasound pressure mapping system using three 1D motion actuators, a HNR needle hydrophone, LabVIEW programming, and MATLAB programming to view ultrasound pressure waves in designated 2D and 3D spaces • First author of a poster titled 3D Mapping of Ultrasonic Pressure through the Ex-Vivo Rat Skull: Methodology and Results (Annual Conference of the Institute for Engineering in Medicine, September 2016)• Third author of a poster titled Noninvasive Mapping of Brain Activations by Transcranial Focused Ultrasound Targeting in Rodents (Minnesota Neuromodulation Symposium, April 2017) • Third author of a poster titled Induction of long-term depression using transcranial focused ultrasound in rat hippocampus (Society for Neuroscience, November 2017)• Fourth author of a publication in preparation in which I performed 3D and 2D scans to calibrate the ultrasound waveform, participated in ultrasound neuromodulation experiments in rats, and helped to develop signal processing codes for the collected electrophysiological data

• The Tissue Engineering and Cell Culture Laboratory at LA Tech University (Dr. Murray) Lab Assistant: Experience with the use of cell culture hoods, incubators, centrifuges, and a liquid nitrogen storage container.Tested different concentrations of carbachol to maximize frequency uptake of calcium in Sh-Ep1 cells with α7 subunit. Measured using epi-fluorescence microscopy and MatLab analysis post experiment. Co-authored a conference paper entitled The Alpha7-Beta2 Nicotinic Receptor and Its Roles in Amyloid Beta Pathology in Alzheimer’s Disease (Southern Biomedical Engineering Conference, March 2016).• The Integrated Neuroscience Imaging Laboratory at LA Tech University (Dr. Murray) Lab Assistant: Experience with in vivo research; specifically mice.Perform behavioral tests on mice including social interaction and tail suspension to determine possible behavioral changes from traumatic brain injuries in mice. Analyzed data from videos taken during tests.• The Brain Dynamics Laboratory at LA Tech University (Dr. Iasemidis)Lab Assistant: Experience with signal processing in time and frequency domains using MatLab.Processed EEG signals and analyzed data from processed EEGs for seizure prediction in epileptic patients by using linear discriminant analysis of the coherence of frequency bands and spectral entropy. Analyzed clinical EEG data and hemodynamic data to develop an algorithm that determines the depth of anesthesia in patients undergoing surgery.

Columbus, In

Worked admissions, cashier of concessions, and assisted/lead child swim classes

Columbus, In

Bag groceries, bring in carts, cashier, stock shelves, and make out orders for supplies

Doctor Of Philosophy - Phd, Biomedical Engineering

Bachelor’S Degree, Biomedical Engineering, Alumnus

Activities and Societies: Delta ChiEarned the Presidential and Bulldog Scholarships