John Mclean Email & Phone Number

Toronto, Ontario, Canada

Toronto, Ontario, Canada

I work with and supervise an exciting young team as we lead the design and development of multiplexed immunoassays from early stage research and prototyping, design and development, verification and validation, risk assessment, and product launch activities. I work cross-functionally with other scientists, hardware/software engineers, manufacturing scientists, and QC/QA/RA to ensure product designs are safe, effective, and marketable.Current areas of focus include:-Preparing for the… Show more I work with and supervise an exciting young team as we lead the design and development of multiplexed immunoassays from early stage research and prototyping, design and development, verification and validation, risk assessment, and product launch activities. I work cross-functionally with other scientists, hardware/software engineers, manufacturing scientists, and QC/QA/RA to ensure product designs are safe, effective, and marketable.Current areas of focus include:-Preparing for the launch of a 6-plex semi-quantitative COVID-19 antibody test with approval through NYSDOH-Health Canada approval for a lung inflammation triage test for healthcare settings-A 4-plex lung transplant cytokine assay Show less

Toronto, Canada Area

Toronto, Canada Area

Collaborated with industry leaders to develop and optimize ultrasensitive, multiplexed immunoassays with clinical and diagnostic applications. I was personally responsible for the development and optimization of quantitative assays used for the detection of clinically relevant biomarkers related to allergy, traumatic brain injury, and pathogenic disease. I utilized 2 cutting edge analysis platforms to analyze clinical samples, including whole blood and serum. I was required to prepare and… Show more Collaborated with industry leaders to develop and optimize ultrasensitive, multiplexed immunoassays with clinical and diagnostic applications. I was personally responsible for the development and optimization of quantitative assays used for the detection of clinically relevant biomarkers related to allergy, traumatic brain injury, and pathogenic disease. I utilized 2 cutting edge analysis platforms to analyze clinical samples, including whole blood and serum. I was required to prepare and present my findings both internally to superiors, and externally to clients. Additionally, I was responsible for manufacturing of various consumables following established standard operating procedures. Show less

Guelph Ontario, Canada

Lead tutorial sessions with 160 students. Responsible for presenting course material, leading discussions and answering student questions. Graded midterms, final examinations and research papers.

Guelph Ontario, Canada

Utilized multiple strain typing techniques including, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to identify the etiological agent of pododermatitis of farmed mink in Canada. Worked as part of a team to develop and optimize a diagnostic quantitative real-time PCR for detection of specific pathogenic bacteria from clinical samples of diseased and healthy animals. Conducted cell-growth assays including growth curves and antibiotic susceptibility testing.

Guelph Ontario, Canada

Screened an expression library of Mycobacterium avium subsp. paratuberculosis proteins for immunoreactive antigens using cow sera for the development of an enzyme-linked immunosorbant assay (ELISA). Utilized protein purification methods, protein assays and Western immunoblotting to identify potential diagnostic proteins.

Master Of Science (M.Sc.), Cell/Cellular And Molecular Biology

Research and development of new methodologies for the isolation and identification of free-living amoeba. Conducted environmental sampling.

Bachelor Of Science (B.Sc.), Microbiology, General

Gained strong grasp of current concepts related to the fields of microbiology, molecular biology and cellular biology. Accomplished.