Jonathan Marvin Email & Phone Number

Current I focus on making intensity-based fluorescent biosensors from GFP and periplasmic binding proteins. When these proteins bind their small molecule ligand (glucose, glutamate, GABA, etc.), fluorescence increases by about 5-fold. We are making sensors for other neurotransmitters by using periplasmic binding proteins that already have the desired specificity and by designing new periplasmic binding proteins to accommodate ligands for which natural binding proteins do not exist. I use both computational protein design and library-based protein design (phage display and high throughout screening).

Listed skills include Protein Chemistry, Biochemistry, Protein Purification, Molecular Biology, and 10 others.