Josh Smith Email & Phone Number

Dublin, Ireland

Greater Boston Area

Prepared and distributed samples for various studies as requested by other chemistry groupsTested and validated historic, newly synthesized, and newly purchased compounds using MS, LC-MS, and NMR Tracked compounds and updated inventory as necessaryPerform various other tasks as required

Greater Boston Area

Assisted in teaching professional masters students modern analytical techniques commonly used in industry.Worked alongside the professor to ensure students gained experience in operating various analytical instrumentation.Helped students learn multiple chromatographic separation techniques including SEC, HIC, and cation exchange.Introduced students to additional immunochemical and proteomic techniques such as common protein assays, one and two dimensional gel electrophoresis, and fast western blotting and imaging.Tested experimental protocols and helped maintain and troubleshoot instrumentation. Prepared solutions, reagents, and samples necessary for the class.

Greater Boston Area

Performed multiple projects focused on the identification of potential candidate cancer biomarkers in human plasma and serum through the use of shotgun proteomics. Developed and optimized chromatographic techniques used to isolate low abundant glycoproteins from biological samples using multidimensional HPLC. Primary focus was on the use of multiple lectin affinty chromatography (MLAC) for capture of glycoproteins in samples. Prepared samples for mass spectrometry identification using in-gel and in-solution trypsin digestion.Identified proteins using LTQ and Orbitrap LC-MS/MS. Compared the effectiveness of ultracentrifugation, gel chromatography, and Exoquick in the isolation of exosomes from human serum. Profiled glycans in exosomes using UPLC

Sydney, Australia

Utlilized shotgun proteomics to identify changes in the proteome profile of a set of human colon cancer cell lines at varying pathological states of colorectal cancer Seeded, passaged, and harvested cells for total protein analysisIdentified proteomic profile of cell lines using LTQ LC-MS/MSHelped identify and analyze glycans in a set of colorectal cancer cell lines using LTQ LC-MS/MS

Greater Boston Area

Synthesized organic compounds that had potential to be drug lead candidatesPurified synthesized compounds using reversed phase chromatography Identified synthesized compounds using LC-MS and NMR