Kaden Huff Email & Phone Number

San Diego, California, United States

•Developed sequencing chemistries and novel products on Illumina’s NGS platforms, advancing applications in oncology, reproductive health, and microbiology. •Lead a study investigating the use of chemical additives to increase PCR yield of products and developed solutions to execute cost-saving measures in on-market products•Interpreted and analyzed sequencing data to determine effectiveness of sequencing chemistry changes •Prepared detailed reports and delivered impactful presentations on findings to drive team decisions.

San Diego, California, United States

•Cloned, produced, and purified plasmid DNA for use in lipid nanoparticle delivery system studies in mouse and non-human primates. Ensured DNA was exceptionally pure and well characterized by quality control before release. •Continually worked on improving process and QC workflow to optimize time management and ensured accuracy of results. Presented findings to the team, discussed solutions, and implemented them independently and with scientist’s guidance•Worked nano-plasmid, MiniCircle, and plasmid DNA backbones as well as unique and challenging secondary structure conformations such as ITR and doggybone DNA•Worked with scientists to implement DNA payload designs to increase expression yield and quality

San Diego, California, United States

• Responsible for the generation of AAV virus for a diverse range of animal studies. Handled the process from plasmid preparation, cell maintenance, transfection, and virus collection. Handled process under tight deadlines and delivered quality product• Mammalian cell culture of suspension and adherent HEK293, HEK001 cells as well as IMR90 cells at scale• Conducted cloning experiments with lead scientists for RnD projects. Troubleshot molecular biology errors independently and with the team. Helped create 25+ unique and difficult-to-assemble constructs within 6 months• Designed cloning strategies using a variety of techniques including restriction enzymes and assembly (Gibson/Infusion). Experienced in software to align and view sequences (Benchling, SnapGene, Geneious)• Extraction of RNA and DNA from animal tissue samples and bacteria• Conducted analysis of viral content with qPCR and DLSS (Stunner)

San Diego, California, United States

San Diego, California, United States

•Conducted analysis of in-process and finished formulations, tracked, and performed stability studies•Supported Senior Scientists in assay work and research for clinical phase and commercial vaccines [VaxChora]•Performed project-specific assays ELISA (TCID50), cell culture, CFU assay, and sample management.•Maintained and passaged mammalian cell line Vero Cells•Conducted Investigatory work on new instrumentation (ESD and NanoSight to help speed along assay development)•Performed small scale lyophilizer run analysis through several typical analytical assays including pH, osmolality, appearance checking, and reconstitution time checks•Assisted with lab management and administrative tasks. Directly assisted in lab supply operations through ordering and contacts with vendors•Ensured compliance with GLP and other regulatory guidelines in lab notebook and practices in the lab

Carlsbad, California

•Worked with lab management in operations, asset management, calibration and preventative maintenance tracking in IVD-regulated lab•Ensured uniformity, reproducibility, and quality in the lab. Maintained compliance with IVD regulations specifically: ISO 13485 and FDA 21 CFR part 820•Was a novice user in a cost/benefit analysis study where I performed three workflows from library preparation to target sequencing on Illumina’s MiSeq, Thermo Fisher’s Chef+S5 system, and Thermo Fisher’s Genexus system•Performed qualifications on Applied Biosystem 96-well Veriti Dx system (PCR machine)•Assisted with inventory tracking and supply logistics•Worked alongside Environmental Health and Safety, Quality Assurance, and Regulatory to ensure the quality management system was followed•Built and reinforced knowledge of best practices in the lab with scientists•Participated in audit preparation and compliance

Greater San Diego Area

• Operated, monitored, and maintained landfill gas migration control/recovery systems (GCMS) on closed and active landfill sites.• Conducted field sampling of surface emissions (GHG: Greenhouse Gas) in compliance with California AB32 regulation. Supervised fellow technicians and subcontractors.• Data recording and analysis. Reviewed data in consultation with supervisor, adjusting gas flow rates from wells, headers, and blowers in GCMS • Recorded and maintains daily field logs reporting data collected, field observations, actions taken, and recommendations.• Performed routine and non-routine repairs and maintenance on GCMS including use of portable saws.• Conducted on-site health and safety meetings with colleagues and subcontractors to ensure safety standards were followed. Actively participated in making safety observations to further the development of the company’s health and safety program.• Reviewed and helped create site specific health and safety programs for San Diego City, County, Alaska and Arizona sites.

Greater San Diego Area

• Responsible for design and execution of experiment in lab and closed city park/trail• Analyzed capability of Miner’s lettuce to uptake or exclude heavy metal arsenic. Experiment replicated conditions of wild Miner’s lettuce thriving in soils containing up to 2% arsenic• Field sampling of soil, calibrated and used XRF (Olympus X-5000) to determine As concentration (ppm)• Evaluated potential exposure of Arsenic in soils to humans

Greater San Diego Area

• Assisted with daily lab tasks including analysis of urine specimens, data entry, aliquoting for testing, reporting results to officers, and reporting results to national lab• Gained experience of US court systems and consistently utilized attention to detail• Acquired chemical knowledge of drugs of abuse including their cross reactivity and the body's metabolites

Greater San Diego Area

• Fundraised and recruited members for a trip to Panama which collected nearly $50k• Provided clinical care for the underserved populations in Pirati and Quebrada Honda• Shadowed 10 doctors in triage, consultation and diagnosis, dentistry, and pharmacy • Analyzed patient data and input into Excel spreadsheets• Created a rice paddy with a team of 30 for native tribe in eastern Panama

Greater San Diego Area

• Led team building exercises and group management during hikes, kayaking, various activities and at camp • Outdoor expertise including knots, map reading, backpacking, and nutrition• Designed and arranged schedules and logistics for trips involving 5-15 people• Exercised time management skills

La Mesa, Ca

I worked as a Camp Counselor for kids aged 5-14. I led campers in onsite and offsite activities and field trips. My main duties were ensuring the safety of children as well as having a positive attitude to share with the campers each day. I took pride engaging with the children and serving as a role model.

Applied Bioinformatics

Bachelor'S Degree, Biology, General

Activities and Societies: University of San Diego Medical Brigades Outdoor Adventures