Liquid enzyme formulation developmentProject Leader

•Worked on butanol fermentation from Eastern redcedar•Hands on experience of several Clostridium strains such as Clostridium beijerinckii DSM 791, •Clostridium beijerinckii DSM 792, Clostridium beijerinckii DSM 6423, Clostridium beijerinckii NCIMB 8052, and Clostridium acetobutylicum ATCC 824•Experience on enzymatic hydrolysis detoxification process•Plan, budget and procure butanol fermentation equipment and supplies•Analyze and interpret experiments results to PIs and collaborators•Hands on biomass pretreatment (SPORL) using Parr Reactor•Biomass compositional analysis, enzymatic hydrolysis from pretreated biomass and enzymes activities test•Knowledge of statistical analysis using SAS program for T-test, Post-hoc analysis, Dunnett test and Plackett–Burman design•Four years of experience on developing GC and HPLC methods and troubleshooting•Supervise undergraduate students on lab techniques, equipment operation and designing/performing experiments

•Gained 4+ years of work experience with syngas fermentation using various strains including monocultures of Alkalibaculum bacchi, Clostridium ragsdalei P11, Clostridium carboxidivorans P7 and a mixed culture of A. bacchi and Clostridium propionicum•Successfully demonstrated characteristics of novel strains A. bacchi in batch syngas fermentation and scaled up fermentations to 3-L and 7-L fermentors under semi-continuous and continuous syngas fermentation •Designed bioreactor setup for continuous syngas fermentation with cell recycle system; successfully operated cell recycle module for syngas fermentation and achieved cell concentration 5.5 g/L•Demonstrated the opportunity of a mixed culture of A. bacchi and C. propionicum syngas fermentation for n-propanol, n-butanol and n-hexanol production•Designed and operated gas-liquid mass transfer experiment on a 7-L continuous stirred tank reactor (CSTR); modeled mass transfer characteristics on the CSTR to guide the working parameters to improve the gas-liquid mass transfer on syngas fermentation•Worked in collaboration with multidisciplinary team to investigate C. carboxidivorans P7 physiological activity during syngas fermentation in a 7-L fermentor •Published four peer-reviewed journal papers•Delivered 16 presentations in international and state meetings/conferences •Documented and transferred techniques for syngas fermentation and butanol fermentation•Operated Parr reactor for biomass pretreatment, conducted biomass composition analysis, enzymatic hydrolysis of pretreated biomass •Experience of butanol fermentation using Clostridium beijerinckii DSM 791 and Clostridium beijerinckii DSM 6423•Prepared project budget and procured lab equipment and supplies•Knowledge of statistical analysis using SAS program for T-test, Post-hoc analysis, Dunnett test and Plackett–Burman design•Supervised undergraduate students on lab techniques, equipment operation and designing/performing experiments

•Working on corn germ oil extraction from aqueous enzymatic treatment•Experience with corn pretreatment for germ separation, milling, drying and size screening•Experience with enzymes such as α-amylase, β-glucosidase, cellulase, proteinase and pectinase preparation for biomass treatment•Hands on corn germ composition analysis such as oil content under rotary evaporation, protein content under Kjeldahl determination, saturate and unsaturated oil content under GC•Tutoring undergraduate student for senior design class

•Working on key scientific and technological program of Tianjin “Bioconversion of 17α-methyl-testosterone for Preparation of Methandienone with Arthrobacter simplex AS 1.94. Mainly focused on extracting of Methandienone from fermentation broth•Experience with aerobic fermentation using fugi and bacteria•Experience with Thin Layer Chromtoography (TLC) analysis