Graduate Research Assistant in the Hummon Lab-The Hummon Research Group develops and applies analytical methods to investigate the proteome and transcriptome in colorectal cancer.PRIMARY PROJECTS• Quantitative comparative proteomic analysis between diploid and aneuploid cancer• Comprehensive comparison of offline bottom-up proteomic sample preparations• Evaluation of the effect of a microRNA cluster on the colon cancer transcriptome and proteome• Validated statistical models of relapse using cell culture models• Developed a clinically applicable genomic test for metastatic potential in colon cancerLABORATORY TECHNIQUES MASTERED• Operating nanoflow UPLC-electrospray mass spectrometers (Thermo Q-Exactive and Orbitrap Velos and AB Sciex 5500 QTRAP)• Bottom-up proteomic workflow (1D gels, in-gel tryptic digest, in-solution tryptic digest, filter-aided sample preparation, sample fractionation, sample clean-up, etc)• MALDI mass spectrometry • Quantitative chemical labeling for proteomics (Dimethyl, iTRAQ, SILAC)• Mammalian cell culture• siRNA/microRNA transfections• qRT-PCR• Software programs: Xcalibur (Thermo), Proteome Discoverer (Thermo), FlexAnalysis (Bruker), Analyst (AB Sciex), Skyline, MaxQuant, Microsoft Office SuiteTEACHING ASSISTANT EXPERIENCE-Laboratory Teaching Assistant for General Chemistry (Fall 2009 and Spring 2010)• Laboratory TA assignment duties: lead students through experiments, give pre-lab lectures, grade lab reports, assist students with post-lab data analysis, grade lecture exams, and oversee student safety