Kiersten Thomas Email & Phone Number

Toronto, ON, CA

Vancouver, British Columbia, Canada

Toronto, Canada Area

Montreal, Canada

• Hypoxia-Induced Epithelial to Mesenchymal Transition (EMT) in ER-Positive Breast CancerCo-supervised by Dr. Etienne Gagnon and Dr. Sylvie Mader
• Comparison of metabolic stressors on ER positive and ER negative breast cancer
• Development of GFP reporter cell lines for Hypoxia Inducible Factor 1 alpha (HIF1a) and Oxygen Dependent Degradation (ODD) by plasmid cloning and lentivirus transduction
• EMT induction by gradual oxygen deprivation with or without estradiol… Show more Hypoxia-Induced Epithelial to Mesenchymal Transition (EMT) in ER-Positive Breast CancerCo-supervised by Dr. Etienne Gagnon and Dr. Sylvie.
• EMT induction by gradual oxygen deprivation with or without estradiol supplementation
• Phenotype analysis by flow cytometry and cell imaging; RT-qPCR and Western Blot analysis of EMT and breast cancer subtype conversion markers Show less

Chalk River, Ontario

• Low Dose Radiation and Immune Checkpoint Combination Therapy for Cancer TreatmentSupervised by Dr. Yi Wang
• Treated mice with alternating radiotherapy and PD-1/PDL1 immunotherapy
• Measured tumour progression and treatment efficacy by in vivo imaging, manual caliper measurements, flow cytometry
• Primary tissue culture of excised metastatic tumor cells
• 3D cell culture and spheroid formation assay

Toronto, Canada Area

• Natural Killer T (NKT) Cell Response to Murine Norovirus (MNV)Supervised by Dr. Thierry Mallevaey
• Generation of recombinant MNV from plasmid through 293T transfection and amplification in RAW264.7 cells
• Plaque assay to determine virus titer
• Co-culture of infected BMDC and NKT cells
• ELISA for cytokine production

• Worked with MSc student Juan Mauricio Umaña in the lab of Dr. Thierry Mallevaey to study Transgenic T-Cell Receptor iNKT Cells for Adoptive Cell Cancer Therapy
• Isolation of natural killer T-cells (NKT) from mouse spleen
• Miniprep plasmid isolation, DNA cloning and transfection
• Retrovirus production in Phoenix™ retroviral packaging cell lines and infection of cultured iNKT cells

Toronto, Canada Area

• Evaluation of Tumour Infiltrating Lymphocytes (TILs) in Breast CancerSupervised by Dr. Marsela Braunstein
• Creation and digital analysis of virtual tissue microarrays (vTMAs) from immunohistochemistry (IHC) stained breast cancer slides
• Creation of machine learning algorithm for semi-automated analysis of breast cancer slides in Definiens Tissue Studio
• Tissue culturing of breast cancer cell lines
• Laser capture microscopy (LCM) and DNA extraction of TILs in formalin-fixed… Show more Evaluation of Tumour Infiltrating Lymphocytes (TILs) in Breast CancerSupervised by Dr. Marsela Braunstein
• Laser capture microscopy (LCM) and DNA extraction of TILs in formalin-fixed paraffin-embedded (FFPE) tumour samples Show less

• Cost-Effectiveness Analysis of Magnetic Resonance Angiography (MRA) in the Assessment of Carotid Artery Stenosis and the Prevention of StrokeSupervised by Dr. Pascal Tyrrell
• Literature search, data collection and statistical analysis using computer-generated models and TreeAge Pro software
• Compared the cost effectiveness of MRA to the standard use of ultrasound for the diagnosis and monitoring of carotid artery stenosis
• Co-authored paper published in The British Journal of… Show more Cost-Effectiveness Analysis of Magnetic Resonance Angiography (MRA) in the Assessment of Carotid Artery Stenosis and the Prevention of StrokeSupervised.
• Co-authored paper published in The British Journal of Radiology (BIR) Show less

Master Of Science (Msc), Molecular Biology

Bachelor Of Science (Bsc), Immunology