Christian Köhler Email & Phone Number

Göttingen, Lower Saxony, Germany

Ghent, Flemish Region, Belgium

Ghent, Belgium

- Production, purification and characterization of Single Domain Antibodies- team leading- scientific lead of Nanobody discovery projects

Mölndal, Sweden

Mechanistic investigations of nuclear hormone receptor activationPublications:Postel, S., Wissler, L., Johansson, C.A., Köhler, C. et al. Quaternary glucocorticoid receptor structure highlights allosteric interdomain communication. Nat Struct Mol Biol (2023). https://doi.org/10.1038/s41594-022-00914-4C. Köhler, G. Carlström, A. Gunnarsson, U. Weininger, S. Tångefjord, V. Ullah, M. Lepistö, U. Karlsson, T. Papavoine, K. Edman, and M. AkkeDynamic allosteric communication pathway directing differential activation of the glucocorticoid receptor.Science Advances 17 Jul 2020 DOI: 10.1126/sciadv.abb5277Köhler C, Carlström G, Tångefjord S, Papavoine T, Lepistö M, Edman K, Akke MBackbone 1H, 13C, and 15N resonance assignments of the ligand binding domain of the human wildtype glucocorticoid receptor and the F602S mutant variant.Biomol NMR Assign. 2018 Apr 17. doi: 10.1007/s12104-018-9820-9Ripa L, Edman K, Dearman M, Edenro G, Hendrickx R, Ullah V, Chang HF, Lepistö M, Chapman D, Geschwindner S, Wissler L, Svanberg P, Lawitz K, Malmberg J, Nikitidis A, Olsson RI, Bird J, Llinas A, Hegelund-Myrbäck T, Berger M, Thorne P, Harrison R, Köhler C, Drmota TDiscovery of a Novel Oral Glucocorticoid Receptor Modulator (AZD9567) with Improved Side Effect Profile.J Med Chem. 2018 Mar 8;61(5):1785-1799Edman K, Hosseini A, Bjursell M K, Aagaard A, Wissler L, Gunnarsson A, Kaminski T, Köhler C, Bäckström S, Jensen T J, Cavallin A, Karlsson U, Nilsson E, Lecina D, Takahashi R, Grebner C, Geschwindner S, Lepistö M, Hogner A C, Guallar VLigand Binding Mechanism in Steroid Receptors: From Conserved Plasticity to DifferentialEvolutionary ConstraintsStructure 2015 23, 1–11Köhler C, Bäckström S, Weininger U, Carlström G, Edman K, Lepistö M,Papavoine T, Akke MNMR characterization of ligand induced dynamic changes in the human wildtype glucocorticoid receptor.oral presentation at ISMAR conference 2015, Shanghai

Illkirch, France

Structural characterization and interaction strudies on small DNA binding domainsPublications:Koehler C, Bonnet J, Stierle M, Romier C, Devys D, Kieffer B.DNA binding by Sgf11 protein affects histone H2B deubiquitination by Spt-Ada-Gcn5-acetyltransferase (SAGA).J Biol Chem. 2014 Mar 28;289(13):8989-99. doi: 10.1074/jbc.M113.500868. Köhler C, Recht R, Quinternet M, de Lamotte F, Delsuc M, Kieffer BAccurate Protein-Peptide Titration Experiments by Nuclear Magnetic Resonance using low-volume samplesSenta Reichelt (ed.) Affinity Chromatography: Methods and Protocols, Methods in Molecular Biology vol. 1286DOI 10.1007/978-1-4939-2447-9_22

Utrecht, Netherlands

Structural and functional investigations on a bacterial antigen to act against meningitis infections Publications: Koehler C, Carlier L, Veggi D, Balducci E, Di Marcello F, Ferrer-Navarro M, Pizza M, Daura X, Soriani M, Boelens R, Bonvin AM.Structural and biochemical characterization of NarE, an iron-containing ADP-ribosyltransferase from Neisseria meningitidis.J Biol Chem. 2011 Apr 29;286(17):14842-51. doi: 10.1074/jbc.M110.193623. Carlier L, Koehler C, Veggi D, Pizza M, Soriani M, Boelens R, Bonvin AM.NMR resonance assignments of NarE, a putative ADP-ribosylating toxin from Neisseria meningitidis.Biomol NMR Assign. 2011 Apr;5(1):35-8. doi: 10.1007/s12104-010-9261-6.

Berlin, Germany

Structural and functional characterization of a protein folding mechanism linked to cholesterol homeostasis, bone formation and alzheimers disease. Publications:Köhler C, Lighthouse JK, Werther T, Andersen OM, Diehl A, Schmieder P, Du J, Holdener BC, Oschkinat H.The structure of MESD45-184 brings light into the mechanism of LDLR family folding.Structure. 2011 Mar 9;19(3):337-48. doi: 10.1016/j.str.2010.12.022.Köhler C, Andersen OM, Diehl A, Krause G, Schmieder P, Oschkinat H.The solution structure of the core of mesoderm development (MESD), a chaperone for members of the LDLR-family.J Struct Funct Genomics. 2006 Dec;7(3-4):131-8. Koehler C, Bishop S, Dowler EF, Schmieder P, Diehl A, Oschkinat H, Ball LJ.Backbone and sidechain 1H, 13C and 15N resonance assignments of the Bright/ARID domain from the human JARID1C (SMCX) protein.Biomol NMR Assign. 2008 Jun;2(1):9-11. doi: 10.1007/s12104-007-9071-7.

Doctor Of Philosophy (Phd), Biochemistry, Magna Cum Laude

PhD Thesis: "Structural and functional investigations on mesodermdevelopment (MESD), a chaperone for receptors of the LDLR-family"

Diploma, Biochemistry

Diploma Thesis: "Metabolic Profiling of cultivated haemopoietic stem cells at different stages of differentiation by NMR spectroscopy"