✅ Independently designed and executed experiments, and performed data analysis and interpretation.✅ Prepared and revised formal reports, patent and IND applications, and presented to senior leadership and executive team.✅ Designed in vivo studies for a variety of liquid and solid xenograft tumor models in immunocompromised mice and managed CRO performance, timelines, and deliverables.✅ Project lead on targeting LIV-1 using CAR T cells to treat all breast cancer subtypes.✅ Co-led a cross-functional project utilizing CRISPR libraries and developed an assay de novo to identify selective clonality in primary T cells.✅ Evaluated CRISPR CAR T cell platform technology against several different targets and indications in vitro and in vivo.✅ Investigated use of lead programs for alternative indications.✅ Developed and tested novel combinatorial approaches to increase in vivo efficacy of CAR T cells in murine tumor models.✅ Managed and mentored two junior research associates on my projects.✅ Collaborated with internal teams on projects to improve cargo expression and translation.✅ Attention to detail, excellent organizational skills and careful record-keeping.

✅ Mammalian Cell Culture and Sterile Technique🔬 Primary human αβ and γδ T cells, HSPCs, and immortalized human cell lines.🧬 Multiple transfection platforms: liposomes, electroporation, and viral-mediated infection. ✅ CAR T-Cell In Vitro and In Vivo Screening💊 Cytotoxicity and LD50 determinations.🧪 Co-culture assays.📊 Cell phenotyping via flow cytometry.🧫 Cytokine secretion profiling.🔍 Exploration of novel synergistic approaches.🐁 Xenograft cancer models in immunocompromised mice.✅ Design, Generation, and Validation of Chimeric Antigen Receptors Against Novel Targets ✅ CRISPR-Cas9 Genome Editing✂️ Guide RNA design and validation.✅ Multi-Color Flow Cytometry and Data Analysis ✅ Viral Vector Design, Titering, and Transduction🦠 Adeno-associated viral vector 6.🧬 HIV-1 lentiviral vector. ✅ Murine Intravenous Injections and Submandibular Plexus Sampling ✅ Protein Characterization📈 SDS PAGE, Western blot, Capillary-based Western (Wes/Jess), ELISA.

CAR-T cell vector design, production and functional analysesCRISPR-Cas9 genome editing in primary human T cells